Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E
| Autor: | Reki Winarto, Raymond R. Tjandrawinata, Ignatius Riwanto, Nugroho Trilaksana |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cyclin E lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Cyclin D Cell cycle Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences 0302 clinical medicine Phaleria macrocarpa medicine Mahkota Dewa E2F lcsh:QH301-705.5 biology Retinoblastoma Cell growth DLBS1425 medicine.disease biology.organism_classification Cell biology 030104 developmental biology lcsh:Biology (General) Cell culture 030220 oncology & carcinogenesis Cancer research biology.protein |
| Zdroj: | Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 4, Pp 280-287 (2017) |
| ISSN: | 2221-1691 |
| Popis: | Objective To prove the molecular mechanisms of Mahkota Dewa ( Phaleria macrocarpa ) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression. Methods In this study, the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa (DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability, the assessment of population profiles of tumor cells in the cell cycle, and the mRNA concentration of p16, p21, p53, cyclin D, cyclin E, and E2F. Results DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line. Inhibition of proliferation was shown by suppression of cell cycle progression. DLBS1425 downregulated cyclin E, a G1 phase regulator gene of cell cycle, in dose-dependent manner without affecting p53–p21 pathway. In the other word, DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway. Conclusions Our results suggest that DLBS1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer. |
| Databáze: | OpenAIRE |
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