Calcium signaling through a transient receptor channel is important for Toxoplasma gondii growth
| Autor: | Nathan M. Chasen, Ivana Y. Kuo, Miryam Andrea Hortua Triana, Karla M. Márquez-Nogueras, Silvia N. J. Moreno |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Science Toxoplasma gondii calcium entry calcium signaling transient receptor channel General Biochemistry Genetics and Molecular Biology Transient receptor potential channel parasitic diseases Extracellular Biology (General) Ion channel Calcium signaling General Immunology and Microbiology biology Chemistry General Neuroscience Endoplasmic reticulum ER calcium General Medicine biology.organism_classification Cell biology Cytosol Medicine Intracellular |
| Zdroj: | eLife, Vol 10 (2021) |
| Popis: | Transient receptor potential (TRP) channels participate in calcium ion (Ca2+) influx and intracellular Ca2+ release. TRP channels have not been studied in Toxoplasma gondii or any other apicomplexan parasite. In this work, we characterize TgGT1_310560, a protein predicted to possess a TRP domain (TgTRPPL-2), and determined its role in Ca2+ signaling in T. gondii, the causative agent of toxoplasmosis. TgTRPPL-2 localizes to the plasma membrane and the endoplasmic reticulum (ER) of T. gondii. The ΔTgTRPPL-2 mutant was defective in growth and cytosolic Ca2+ influx from both extracellular and intracellular sources. Heterologous expression of TgTRPPL-2 in HEK-3KO cells allowed its functional characterization. Patching of ER-nuclear membranes demonstrates that TgTRPPL-2 is a non-selective cation channel that conducts Ca2+. Pharmacological blockers of TgTRPPL-2 inhibit Ca2+ influx and parasite growth. This is the first report of an apicomplexan ion channel that conducts Ca2+ and may initiate a Ca2+ signaling cascade that leads to the stimulation of motility, invasion, and egress. TgTRPPL-2 is a potential target for combating toxoplasmosis. |
| Databáze: | OpenAIRE |
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