Complexome profiling on the Chlamydomonas lpa2 mutant reveals insights into PSII biogenesis and new PSII associated proteins
| Autor: | Matthieu Mustas, Timo Mühlhaus, Benedikt Venn, Frederik Sommer, Michael Schroda, Benjamin Spaniol, Lara Schake, Stefan Geimer, Yves Choquet, Julia Lang, Francis-André Wollman |
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| Přispěvatelé: | Technische Universität Kaiserslautern (TU Kaiserslautern), Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Biologie du chloroplaste et perception de la lumière chez les micro-algues, Institut de biologie physico-chimique (IBPC (FR_550)), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Universität Bayreuth |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
[SDV.BIO]Life Sciences [q-bio]/Biotechnology Physiology CP43 photosystem I antenna Mutant Light-Harvesting Protein Complexes Plant Science macromolecular substances 01 natural sciences Thylakoids 03 medical and health sciences thylakoid membranes Photosynthesis complexome profilings 030304 developmental biology mass spectrometry 0303 health sciences BN-PAGE biology ATP synthase Cytochrome b6f complex Chemistry AcademicSubjects/SCI01210 Chlamydomonas complexome profiling food and beverages Photosystem II Protein Complex photosystem II subunits biology.organism_classification Research Papers 3. Good health Thylakoid Chlorophyll breakdown biology.protein Biophysics Biogenesis comigration profiles photosystem II biogenesis 010606 plant biology & botany Photosynthesis and Metabolism |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2021, ⟨10.1093/jxb/erab390⟩ |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erab390⟩ |
| Popis: | We identify three novel proteins potentially associated with PSII and a role for auxiliary factor LPA2 in catalysing a step required for stable PSII assembly beyond PSII monomers in Chlamydomonas. While the composition and function of the major thylakoid membrane complexes are well understood, comparatively little is known about their biogenesis. The goal of this work was to shed more light on the role of auxiliary factors in the biogenesis of photosystem II (PSII). Here we have identified the homolog of LOW PSII ACCUMULATION 2 (LPA2) in Chlamydomonas. A Chlamydomonas reinhardtii lpa2 mutant grew slower in low light, was hypersensitive to high light, and exhibited aberrant structures in thylakoid membrane stacks. Chlorophyll fluorescence (Fv/Fm) was reduced by 38%. Synthesis and stability of newly made PSII core subunits D1, D2, CP43, and CP47 were not impaired. However, complexome profiling revealed that in the mutant CP43 was reduced to ~23% and D1, D2, and CP47 to ~30% of wild type levels. Levels of PSI and the cytochrome b6f complex were unchanged, while levels of the ATP synthase were increased by ~29%. PSII supercomplexes, dimers, and monomers were reduced to ~7%, ~26%, and ~60% of wild type levels, while RC47 was increased ~6-fold and LHCII by ~27%. We propose that LPA2 catalyses a step during PSII assembly without which PSII monomers and further assemblies become unstable and prone to degradation. The LHCI antenna was more disconnected from PSI in the lpa2 mutant, presumably as an adaptive response to reduce excitation of PSI. From the co-migration profiles of 1734 membrane-associated proteins, we identified three novel putative PSII associated proteins with potential roles in regulating PSII complex dynamics, assembly, and chlorophyll breakdown. |
| Databáze: | OpenAIRE |
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