Evaluation of reduced allergenicity of deamidated gliadin in a mouse model of wheat-gliadin allergy using an antibody prepared by a peptide containing three epitopes
| Autor: | Hitoshi Kumagai, Makoto Akao, Masae Sugai, Karin Yasuda, Hitomi Kumagai, Shiori Shimizu, Ryosuke Abe, Kazuhiro Chiba, Yohei Okada |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Wheat Hypersensitivity medicine.disease_cause Immunoglobulin E digestive system Epitope Gliadin Epitopes Mice Allergen medicine Animals Humans Deamidation Anaphylaxis Triticum Mice Inbred BALB C Intestinal permeability medicine.diagnostic_test biology Chemistry Radioallergosorbent test Hydrolysis nutritional and metabolic diseases food and beverages General Chemistry medicine.disease digestive system diseases Intestines Biochemistry Deamination biology.protein General Agricultural and Biological Sciences Wheat allergy |
| Zdroj: | Journal of agricultural and food chemistry. 62(13) |
| ISSN: | 1520-5118 |
| Popis: | Gliadin is the principal allergen of wheat-dependent exercise-induced anaphylaxis (WDEIA). The primary structure of IgE-binding epitopes in wheat gliadin includes tandem sequencing sites of glutamine residues. Therefore, deamidation would be an effective approach to reduce the allergenicity of wheat proteins. In our previous study, we deamidated wheat gliadin without causing peptide-bond hydrolysis or polymerization by use of carboxylated cation-exchange resins, and we found that the deamidated gliadin scarcely reacted with the sera of patients radioallergosorbent test (RAST)-positive to wheat. In this study, we examined the allergenicity of deamidated gliadin in a mouse model of wheat-gliadin allergy. Oral administration of deamidated gliadin to gliadin-sensitized mice suppressed enhancement in intestinal permeability, serum allergen level, serum allergen-specific IgE level, mast-cell-surface expression of FceRI, and serum and intestinal histamine levels. Our results indicate that gliadin deamidated with no peptide-bond hydrolysis by cation-exchange resins has low allergenicity even under in vivo conditions. |
| Databáze: | OpenAIRE |
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