The Relationship between Neurite Density Measured with Confocal Microscopy in a Cleared Mouse Brain and Metrics Obtained from Diffusion Tensor and Diffusion Kurtosis Imaging
| Autor: | Hidekazu Yoshizawa, Shigeki Aoki, Kazuhiko Tagawa, Koji Kamagata, Hitoshi Okazawa, Ryo Ueda, Kanako Sato, Masaaki Hori, Eri Arikawa-Hirasawa, Ayato Hayashi, Kohske Takahashi, Hisaaki Ochi, Aurelien Kerever, Suguru Yokosawa, Yosuke Otake, Ryusuke Irie |
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| Rok vydání: | 2018 |
| Předmět: |
mice
Neurite Confocal histocytological preparation techniques Anterior commissure confocal microscopy 030218 nuclear medicine & medical imaging law.invention Diffusion 03 medical and health sciences 0302 clinical medicine Nuclear magnetic resonance Confocal microscopy law Fractional anisotropy Neurites Animals Medicine Radiology Nuclear Medicine and imaging Diffusion Kurtosis Imaging diffusion magnetic resonance imaging Microscopy Confocal business.industry Brain Water non-Gaussian mutant strains Diffusion Tensor Imaging Kurtosis Anisotropy business Major Paper 030217 neurology & neurosurgery Diffusion MRI |
| Zdroj: | Magnetic Resonance in Medical Sciences |
| ISSN: | 1880-2206 1347-3182 |
| DOI: | 10.2463/mrms.mp.2017-0031 |
| Popis: | Purpose Diffusional kurtosis imaging (DKI) enables sensitive measurement of tissue microstructure by quantifying the non-Gaussian diffusion of water. Although DKI is widely applied in many situations, histological correlation with DKI analysis is lacking. The purpose of this study was to determine the relationship between DKI metrics and neurite density measured using confocal microscopy of a cleared mouse brain. Methods One thy-1 yellow fluorescent protein 16 mouse was deeply anesthetized and perfusion fixation was performed. The brain was carefully dissected out and whole-brain MRI was performed using a 7T animal MRI system. DKI and diffusion tensor imaging (DTI) data were obtained. After the MRI scan, brain sections were prepared and then cleared using aminoalcohols (CUBIC). Confocal microscopy was performed using a two-photon confocal microscope with a laser. Forty-eight ROIs were set on the caudate putamen, seven ROIs on the anterior commissure, and seven ROIs on the ventral hippocampal commissure on the confocal microscopic image and a corresponding MR image. In each ROI, histological neurite density and the metrics of DKI and DTI were calculated. The correlations between diffusion metrics and neurite density were analyzed using Pearson correlation coefficient analysis. Results Mean kurtosis (MK) (P = 5.2 × 10-9, r = 0.73) and radial kurtosis (P = 2.3 × 10-9, r = 0.74) strongly correlated with neurite density in the caudate putamen. The correlation between fractional anisotropy (FA) and neurite density was moderate (P = 0.0030, r = 0.42). In the anterior commissure and the ventral hippocampal commissure, neurite density and FA are very strongly correlated (P = 1.3 × 10-5, r = 0.90). MK in these areas were very high value and showed no significant correlation (P = 0.48). Conclusion DKI accurately reflected neurite density in the area with crossing fibers, potentially allowing evaluation of complex microstructures. |
| Databáze: | OpenAIRE |
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