Alterations in gene array patterns in dendritic cells from aged humans
| Autor: | Jia Ning Cao, Zhenyu Jia, Sudhir Gupta, Edward Sharman, Anshu Agrawal |
|---|---|
| Přispěvatelé: | Messaoudi, Ilhem |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Aging Gene Expression lcsh:Medicine Adaptive Immunity Animal Cells Gene expression Molecular Cell Biology 80 and over Cluster Analysis Innate 2.1 Biological and endogenous factors Aetiology lcsh:Science Aged 80 and over Multidisciplinary Age Factors Cell migration Acquired immune system Healthy Volunteers medicine.anatomical_structure Female medicine.symptom Cellular Types Research Article Biotechnology Adult General Science & Technology T cell Immune Cells 1.1 Normal biological development and functioning Immunology Antigen-Presenting Cells Inflammation and over Biology Young Adult Immune system Immunity Underpinning research medicine Genetics Humans Gene Aged Gene Expression Profiling Inflammatory and immune system lcsh:R G1 Phase Biology and Life Sciences Cell Biology Dendritic Cells Immunity Innate Gene Expression Regulation lcsh:Q Transcriptome |
| Zdroj: | PLoS ONE, Vol 9, Iss 9, p e106471 (2014) PloS one, vol 9, iss 9 PLoS ONE Cao, JN; Agrawal, A; Sharman, E; Jia, Z; & Gupta, S. (2014). Alterations in gene array patterns in dendritic cells from aged humans. PLoS ONE, 9(9). doi: 10.1371/journal.pone.0106471. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/69b1s01p |
| ISSN: | 1932-6203 |
| Popis: | © 2014 Cao et al. Dendritic cells (DCs) are major antigen-presenting cells that play a key role in initiating and regulating innate and adaptive immune responses. DCs are critical mediators of tolerance and immunity. The functional properties of DCs decline with age. The purpose of this study was to define the age-associated molecular changes in DCs by gene array analysis using Affymatrix GeneChips. The expression levels of a total of 260 genes (1.8%) were significantly different (144 down-regulated and 116 upregulated) in monocyte-derived DCs (MoDCs) from aged compared to young human donors. Of the 260 differentially expressed genes, 24% were down-regulated by more than 3-fold, suggesting that a large reduction in expression occurred for a notable number of genes in the aged. Our results suggest that the genes involved in immune response to pathogens, cell migration and T cell priming display significant age-related changes. Furthermore, downregulated genes involved in cell cycle arrest and DNA replication may play a critical role in aging-associated genetic instability. These changes in gene expression provide molecular based evidence for age-associated functional abnormalities in human DCs that may be responsible for the defects in adaptive immunity observed in the elderly. |
| Databáze: | OpenAIRE |
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