MONITORING OF WATERMELON MOSAIC VIRUS 2 IN AGRIECOSYSTEMS OF KYIV AND POLTAVA REGIONS
| Jazyk: | angličtina |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Indirect elisa
моніторинг Zucchini yellow mosaic virus biology вірус мозаїки кавуна 2 lcsh:Biotechnology food and beverages Standard methods biology.organism_classification Virology Virus lcsh:TP248.13-248.65 Watermelon mosaic virus 2 агроценози Cucurbitaceae Pathogen Mixed infection змішана вірусна інфекція |
| Zdroj: | Mìkrobìologìâ ì Bìotehnologìâ, Vol 0, Iss 4(24), Pp 55-62 (2013) |
| ISSN: | 2307-4663 2076-0558 |
| Popis: | Aim. Monitoring of Watermelon mosaic virus 2 in agriecosystems of Kyiv and Poltava regions. Methods. Plant samples were analyzed using DAS- and indirect ELISA for presence of viral antigens. ELISA samples were prepared by standard methods. For Watermelon mosaic virus 2 (WMV-2) diagnostics, commercial antisera of INRA (France), Agdia (England) and Loewe (Germany) were used. Results. Following 9-year monitoring of Ukrainian agriecosystems we have demonstrated periodically changeable circulation of Watermelon mosaic virus 2 on plants of Cucurbitaceae family. It was determined that, in Ukraine, WMV 2-infected plants were typical only for open ground conditions. Watermelon mosaic virus 2 circulates mainly in form of mixed infection with Zucchini yellow mosaic virus, Cucumber mosaic virus or Cucurbit aphid-born yellows virus. Conclusions. In 2005, after nearly 30 years of absence, WMV-2 has been again detected on plants of Cucurbitaceae family in open-ground agriecosystems of Kyiv and Poltava regions of Ukraine. During the 9-year monitoring, WMV-2 has not been found at all on watermelon plants, though previously this pathogen was widely spread on watermelon plantings. In addition, it should be noticed we have never detected WMV-2 in glasshouse crops. Therefore in Ukraine WMV-2 remains a pathogen infecting exclusively the plants cultivated under open-ground conditions. |
| Databáze: | OpenAIRE |
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