Hospital outbreak caused by Klebsiella pneumoniae producing KPC-2 β-lactamase resistant to colistin
| Autor: | Danai Sofianou, K. Vasilakos, Konstantina Kontopoulou, Asimoula Koteli, M. Kriti, Efthimia Protonotariou, Eleni Antoniadou |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Genotype Klebsiella pneumoniae medicine.medical_treatment Tigecycline Drug resistance Biology Polymerase Chain Reaction beta-Lactamases Disease Outbreaks Microbiology Bacterial Proteins Drug Resistance Multiple Bacterial polycyclic compounds medicine Humans Antibacterial agent Cross Infection Greece Colistin General Medicine Middle Aged biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Virology Hospitals Anti-Bacterial Agents Klebsiella Infections Ciprofloxacin Infectious Diseases Carbapenems Beta-lactamase Female Gentamicin medicine.drug |
| Zdroj: | Journal of Hospital Infection. 76:70-73 |
| ISSN: | 0195-6701 |
| Popis: | We describe a hospital outbreak caused by colistin-resistant Klebsiella pneumoniae producing KPC-2 beta-lactamase in two distinct medical centres. Seven clinical isolates of K. pneumoniae exhibiting resistance to carbapenems were collected from patients with hospital-acquired infection. All isolates were phenotypically positive for carbapenemase activity but negative for metallo-beta-lactamase production. PCR analysis using specific primers for bla(KPC), bla(SHV), bla(TEM) and bla(CTX-M) demonstrated that all clinical strains of K. pneumoniae from hospital A and one isolate from hospital B were genetically related and carried bla(KPC-2) in addition to bla(SHV-12). In contrast, the remaining isolate carried bla(S)(HV-5) with bla(K)(PC-2) and yielded a different profile. These results indicate the clonal spread of KPC producers between hospitals as well as the acquisition of KPC genes by different K. pneumoniae strains. All isolates were resistant to carbapenems, beta-lactams, ciprofloxacin, aminoglycosides and colistin, but intermediately susceptible to tigecycline and susceptible to gentamicin. The infection was fatal in five cases. The emergence of colistin-resistant K. pneumoniae possessing bla(KPC)(-2) underscores the implementation of strict control measures to prevent their dissemination of these organisms in hospitals. |
| Databáze: | OpenAIRE |
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