Murine Gammaherpesvirus (MHV-68) Transforms Cultured Cells in vitro
| Autor: | Veronika Mrázová, Miroslava Šupolíková, Veronika Lachová, Marcela Kúdelová, B. Lapuníková, F. Golais, Tatiana Betakova |
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| Rok vydání: | 2015 |
| Předmět: |
Rhadinovirus
Fluorescent Antibody Technique Biology Virus Replication Immunofluorescence Polymerase Chain Reaction 3T3 cells Mice chemistry.chemical_compound Virus antigen Virology medicine Animals Antigens Viral Cells Cultured Actin Cell Line Transformed medicine.diagnostic_test Fibroblasts Cell Transformation Viral Phenotype Molecular biology In vitro Virus Latency Infectious Diseases medicine.anatomical_structure chemistry Cell culture NIH 3T3 Cells DNA |
| Zdroj: | Intervirology. 58:69-72 |
| ISSN: | 1423-0100 0300-5526 |
| DOI: | 10.1159/000370071 |
| Popis: | Human dermal fibroblasts and mouse NIH/3T3 cells acquired the transformed phenotype (‘criss-cross' pattern of growth) after infection with ultraviolet-irradiated murine gammaherpesvirus (MuHV-4 strain 68; MHV-68). These cells with changed phenotype could be serially cultured for 5-6 passages (35-40 days), and then they entered into crisis and most of them died. In a small number of cultures, however, foci of newly transformed cells appeared from which two stable cell lines were derived. After 6-9 cell culture passages of the MHV-68 transformed cell lines, MHV-68 DNA and virus antigen could be detected by PCR and immunofluorescence assay along with the disappearance of actin bundles, indicating that both transformed cell lines might be oncogenic. |
| Databáze: | OpenAIRE |
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