Comparative medical characteristics of ZDF‐T2DM rats during the course of development to late stage disease
| Autor: | Hu Jingwen, Shuaiyao Lu, Guang-Long Liu, Yun-Li Pang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Zucker diabetic fatty rats endocrine system diseases type 2 diabetes mellitus medicine.medical_treatment 0211 other engineering and technologies clinical indicators 02 engineering and technology Type 2 diabetes 03 medical and health sciences Insulin resistance Internal medicine 021105 building & construction medicine molecular expression Glycogen synthase Adiponectin biology business.industry Insulin Type 2 Diabetes Mellitus nutritional and metabolic diseases General Medicine Original Articles medicine.disease IRS2 IRS1 030104 developmental biology Endocrinology biology.protein histopathology Original Article business |
| Zdroj: | Animal Models and Experimental Medicine |
| ISSN: | 2576-2095 2096-5451 |
| Popis: | Background There are few reports on the comparative medical characteristics of type 2 diabetes models in late stage. An analysis of comparative medical characteristics of Zucker diabetic fatty type 2 diabetes mellitus (ZDF‐T2DM) rats during the course of development to late stage disease was performed. Methods In this study, ZDF rats were fed with high‐sugar and high‐fat diets to raise the fasting blood glucose, and develop of type 2 diabetes. At the late stage of T2DM, the preliminary comparative medical characteristics of the T2DM model were analyzed through the detection of clinical indicators, histopathology, related cytokine levels, and insulin‐related signaling molecule expression levels. Results In the T2DM group, the fasting blood glucose was higher than 6.8 mmol/L, the serum insulin, leptin, and adiponectin levels were significantly decreased, and glucose intolerance and insulin resistance were measured as clinical indicators. Regarding pathological indicators, a large number of pancreatic islet cells showed the reduction of insulin secretion, resulting in damaged glycogen synthesis and liver steatosis. At the molecular level, the insulin signal transduction pathway was inhibited by decreasing the insulin receptor substrate 1 (IRS1), insulin receptor substrate 2 (IRS2), phosphatidylinositol 3 kinase (PI3K), and glycogen synthesis kinase 3β (GSK‐3β) expression levels. Conclusion The results show that the ZDF/T2DM rats have typical clinical, histopathological, and molecular characteristics of human T2DM and thus can be used as an effective model for T2DM drug development and treatment of advanced T2DM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text