Alginate-based hydrogels functionalised at the nanoscale using layer-by-layer assembly for potential cartilage repair
| Autor: | Piergiorgio Gentile, C. Ghione, Ana Marina Ferreira, Paul V. Hatton, Aileen Crawford |
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| Rok vydání: | 2017 |
| Předmět: |
Cartilage
Articular food.ingredient Alginates Biomedical Engineering Biocompatible Materials Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Gelatin chemistry.chemical_compound food Glucuronic Acid Polyamines medicine Animals General Materials Science chemistry.chemical_classification Chemistry Hexuronic Acids Cartilage Regeneration (biology) Biomolecule Layer by layer Hydrogels Adhesion 021001 nanoscience & nanotechnology Nanostructures 0104 chemical sciences medicine.anatomical_structure Chemical engineering Self-healing hydrogels Genipin Cattle 0210 nano-technology |
| Zdroj: | Biomaterials Science. 5:1922-1931 |
| ISSN: | 2047-4849 2047-4830 |
| DOI: | 10.1039/c7bm00525c |
| Popis: | Injuries to articular cartilage are frequently difficult to repair, in part because of the poor regenerative capacity of this tissue. To date, no successful system for complete regeneration of the most challenging cartilage defects has been demonstrated. The aim of this work was to develop functionalised hydrogels at the nanoscale by Layer-by-Layer (LbL) assembly to promote cartilage healing. Hydrogels, based on sodium alginate (NaAlg) and gelatin (G), were prepared by an external gelation method consisting of CaCl2 diffusion and genipin addition for G crosslinking. Successively, hydrogels were coated with G to obtain a positive charge on the surface, then functionalised by LbL assembly to create 16 nanolayers, based on poly(styrene sulfonate)/poly(allyl amine) (PSS/PAH), including a specific peptide sequence (CTATVHL) and transforming growth factors β1 (TGF-β1). Physico-chemical properties were evaluated by XPS, ATR-FTIR and rheological analyses while in vitro cytocompatibility was studied using bovine articular chondrocytes (BAC). XPS spectra showed N1s and S2p peaks, indicating that PAH and PSS have been introduced with success. ATR-FTIR indicated the specific PAH and PSS absorption peaks. Finally, the biomolecule incorporation influenced positively the processes of BAC adhesion and proliferation, and glycosamynoglycan secretion. The functionalised alginate-based hydrogels described here are ideally suited to chondral regeneration in terms of their integrity, stability, and cytocompatibility. |
| Databáze: | OpenAIRE |
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