Spatially-resolved protein surface microsampling from tissue sections using liquid extraction surface analysis
| Autor: | Michel Salzet, Benoit Fatou, Annie Desmons, Maxence Wisztorski, Isabelle Fournier, Jean-Pascal Gimeno, Julien Franck, Jusal Quanico |
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| Přispěvatelé: | Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Site de Recherche Intégrée en Cancérologie (SIRIC-ONCOLille), Université de Lille, Sciences et Technologies-Université de Lille, Sciences Humaines et Sociales-Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université Lille Nord de France (COMUE)-UNICANCER-Université Lille Nord de France (COMUE)-UNICANCER-Cancéropole Nord-Ouest-Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Université de Lille-UNICANCER-Université de Lille-UNICANCER-Cancéropole Nord-Ouest-Université de Lille, Droit et Santé-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), SALZET, Michel |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Proteomics
0301 basic medicine Surface (mathematics) Technology Tissue microproteomics Resolution (mass spectrometry) [SDV]Life Sciences [q-bio] Surface sampling 01 natural sciences Biochemistry 03 medical and health sciences Tandem Mass Spectrometry Animals Humans Molecular Biology Chromatography High Pressure Liquid Brain Chemistry Reproducibility Chromatography Chemistry Spatially resolved LESA 010401 analytical chemistry Extraction (chemistry) Proteins Rats 0104 chemical sciences Characterization (materials science) [SDV] Life Sciences [q-bio] Protein micro-extraction 030104 developmental biology Tissue sections Biological system |
| Zdroj: | Proteomics Proteomics, Wiley-VCH Verlag, 2016, 16 (11-12), pp.1622-1632. ⟨10.1002/pmic.201500508⟩ Proteomics, 2016, 16 (11-12), pp.1622-1632. ⟨10.1002/pmic.201500508⟩ |
| ISSN: | 1615-9853 1615-9861 |
| DOI: | 10.1002/pmic.201500508⟩ |
| Popis: | International audience; Tissue microenvironment characterization presents a challenge for a better understanding of the full complexity of a pathology. Unfortunately, making a precise "picture" of the disease needs an efficient microsampling method coupled to an accurate localization for performing region-dependent proteomics. Here, we present a method that enables rapid and reproducible extraction of proteins from a tissue section to analyze a specific region at a millimeter scale. The method used a liquid-microjunction extraction with conventional detergent solution for proteomics analysis. We successfully performed immunoblotting experiments and showed the possibility to retrieve and identify more than 1400 proteins from a 1-mm diameter spot size on tissue sections with a high degree of reproducibility both qualitatively and quantitatively. Moreover, the small size of the extracted region achieved by this sampling method allows the possibility to perform multiple extractions on different tissue section points. Ten points on a sagittal rat brain tissue section were analyzed and the measured proteins clearly distinguished the different parts of the brain, thus permitting precise functional mapping. We thus demonstrate that with this technology, it is possible to map the tissue microenvironment and gain an understanding of the molecular mechanisms at millimeter resolution. |
| Databáze: | OpenAIRE |
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