Structure and evolution of the small subunit ribosomal RNA gene of Crithidia fasciculata
| Autor: | Michael W. Gray, James C. Collings, Murray N. Schnare |
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| Rok vydání: | 1986 |
| Předmět: |
Sequence analysis
Molecular Sequence Data DNA Ribosomal Molecular evolution Crithidia parasitic diseases RNA Ribosomal 18S Genetics Animals Cloning Molecular Ribosomal DNA Gene Phylogeny Crithidia fasciculata Base Sequence biology fungi Protein primary structure Hydrogen Bonding DNA Restriction Enzymes General Medicine Ribosomal RNA biology.organism_classification Biological Evolution Genes RNA Ribosomal Nucleic Acid Conformation |
| Zdroj: | Current Genetics. 10:405-410 |
| ISSN: | 1432-0983 0172-8083 |
| Popis: | We present the cloning and sequence analysis of the nuclear-encoded Crithidia fasciculata small subunit (SSU) rRNA gene, the longest (2,206 bp) such gene yet characterized by direct sequence analysis. Much of the sequence can be folded to fit a phylogenetically conserved secondary structure model, with the additional length of this gene being accommodated within discrete variable domains that are present in eukaryotic SSU rRNAs. On the basis of sequence comparisons, we conclude that Crithidia contains the most highly diverged SSU rRNA described to date among the eukaryotes, and therefore represents one of the earliest branchings within the eukaryotic primary kingdom. |
| Databáze: | OpenAIRE |
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