The Influence of Palmatine Isolated from Berberis sibirica Radix on Pentylenetetrazole-Induced Seizures in Zebrafish

Autor:	Katarzyna Stępnik, Waldemar A. Turski, Wietske van der Ent, Kinga Gawel, Dominik Tarabasz, Dorota Nieoczym, Wirginia Kukula-Koch, Camila V. Esguerra, Nancy Saana Banono
Rok vydání:	2020
Předmět:	medicine.medical_treatment Berberis sibirica radix complex mixtures Berberidaceae 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Berberine berberine palmatine medicine Radix EEG Isoquinoline Zebrafish lcsh:QH301-705.5 030304 developmental biology seizures 0303 health sciences biology Traditional medicine pentylenetetrazole Palmatine General Medicine biology.organism_classification zebrafish 3. Good health Anticonvulsant chemistry lcsh:Biology (General) anticonvulsant activity Palm locomotor activity isolation 030217 neurology & neurosurgery
Zdroj:	Cells Cells, Vol 9, Iss 1233, p 1233 (2020) Volume 9 Issue 5
ISSN:	2073-4409
DOI:	10.3390/cells9051233
Popis:	Palmatine (PALM) and berberine (BERB) are widely identified isoquinoline alkaloids among the representatives of the Berberidaceae botanical family. The antiseizure activity of BERB was shown previously in experimental epilepsy models. We assessed the effect of PALM in a pentylenetetrazole (PTZ)-induced seizure assay in zebrafish, with BERB as an active reference compound. Both alkaloids were isolated from the methanolic root extract of Berberis sibirica by counter-current chromatography, and their ability to cross the blood&ndash brain barrier was determined via quantitative structure&ndash activity relationship assay. PALM exerted antiseizure activity, as confirmed by electroencephalographic analysis, and decreased c-fos and bdnf levels in PTZ-treated larvae. In a behavioral assay, PALM dose-dependently decreased PTZ-induced hyperlocomotion. The combination of PALM and BERB in ED16 doses revealed hyperadditive activity towards PTZ-induced hyperlocomotion. Notably, we have indicated that both alkaloids may exert their anticonvulsant activity through different mechanisms of action. Additionally, the combination of both alkaloids in a 1:2.17 ratio (PALM: BERB) mimicked the activity of the pure extract, which indicates that these two active compounds are responsible for its anticonvulsive activity. In conclusion, our study reveals for the first time the anticonvulsant activity of PALM and suggests the combination of PALM and BERB may have higher therapeutic value than separate usage of these compounds.
Databáze:	OpenAIRE
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