Down-regulation of acyl-CoA oxidase gene expression and increased NF-κB activity in etomoxir-induced cardiac hypertrophy
| Autor: | Manuel Vázquez Carrera, Manuel Merlos, Àgatha Cabrero, Juan C. Laguna |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Blood Glucose
Male Receptors Steroid medicine.medical_specialty Receptors Retinoic Acid Down-Regulation Receptors Cytoplasmic and Nuclear Peroxisome proliferator-activated receptor Cardiomegaly QD415-436 Biology medicine.disease_cause PPAR Biochemistry Mice chemistry.chemical_compound Endocrinology Internal medicine Gene expression medicine Animals Acyl-CoA oxidase RNA Messenger Enzyme Inhibitors Beta oxidation chemistry.chemical_classification Myocardium Glutathione peroxidase NF-kappa B Nuclear Proteins Heart Cell Biology DNA-Binding Proteins Oxidative Stress COUP Transcription Factors Retinoid X Receptors chemistry Epoxy Compounds Acyl-CoA Oxidase Carnitine palmitoyltransferase I nuclear factor κB Etomoxir Oxidative stress Transcription Factors |
| Zdroj: | Journal of Lipid Research, Vol 44, Iss 2, Pp 388-398 (2003) |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.m200294-jlr200 |
| Popis: | Activation of nuclear factor-kappaB (NF-kappaB) is required for hypertrophic growth of cardiomyocytes. Etomoxir is an irreversible inhibitor of carnitine palmitoyltransferase I (CPT-I) that activates peroxisome proliferator-activated receptor alpha (PPARalpha) and induces cardiac hypertrophy through an unknown mechanism. We studied the mRNA expression of genes involved in fatty acid oxidation in the heart of mice treated for 1 or 10 days with etomoxir (100 mg/kg/day). Etomoxir administration for 1 day significantly increased (4.4-fold induction) the mRNA expression of acyl-CoA oxidase (ACO), which catalyzes the rate-limiting step in peroxisomal beta-oxidation. In contrast, etomoxir treatment for 10 days dramatically decreased ACO mRNA levels by 96%. The reduction in ACO expression in the hearts of 10-day etomoxir-treated mice was accompanied by an increase in the mRNA expression of the antioxidant enzyme glutathione peroxidase and the cardiac marker of oxidative stress bax. Moreover, the activity of the redox-regulated transcription factor NF-kappaB was increased in heart after 10 days of etomoxir treatment. Overall, the findings here presented show that etomoxir treatment may induce cardiac hypertrophy via increased cellular oxidative stress and NF-kappaB activation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|