Radioimmunoassay of rat leptin: sexual dimorphism reversed from humans

Autor: John E. Hale, Peter J. Havel, Bridgette Schoner, Wendy M. Mueller, Michael Landt, Ronald L. Gingerich, Mark L. Heiman
Rok vydání: 1998
Předmět:
Leptin
Male
Medical Biotechnology
Clinical Biochemistry
Adipose tissue
Medical Biochemistry and Metabolomics
Cardiovascular
Rats
Sprague-Dawley
Mice
chemistry.chemical_compound
Adipocyte
Blood plasma
Adipocytes
Receptor
General Clinical Medicine
Cells
Cultured
Inbred F344
Sex Characteristics
Cultured
digestive
oral
and skin physiology
Radioimmunoassay
Stroke
Zucker
Adipose Tissue
Hypothalamus
Female
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
Cells
Clinical Sciences
Biology
Sensitivity and Specificity
Species Specificity
Internal medicine
medicine
Animals
Humans
Obesity
Nutrition
Analysis of Variance
Biochemistry (medical)
Proteins
Reproducibility of Results
medicine.disease
Rats
Inbred F344
Rats
Rats
Zucker
Endocrinology
chemistry
Protein Biosynthesis
Sprague-Dawley
Zdroj: Landt, M; Gingerich, RL; Havel, PJ; Mueller, WM; Schoner, B; Hale, JE; et al.(1998). Radioimmunoassay of rat leptin: sexual dimorphism reversed from humans.. Clinical chemistry, 44(3), 565-570. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/6st0z7v4
Clinical chemistry, vol 44, iss 3
Scopus-Elsevier
ISSN: 1530-8561
0009-9147
DOI: 10.1093/clinchem/44.3.565
Popis: Adipose tissue secretes leptin, which interacts with receptors in the hypothalamus. In rodent models of obesity, leptin increases metabolism and decreases food intake, which helps to maintain normal body composition. Accurate and precise methods to quantitate circulating leptin concentrations are needed for physiological studies. We developed an RIA to measure leptin in rat plasma, serum, or adipocyte culture fluids. The working range of the assay, defined by the detection limit and the highest calibrator, was 0.5–50 μg/L. Recovery of 1.6–11.6 μg/L leptin added to serum was 92–103%. The rat leptin RIA correlated well with a previously developed mouse RIA when rat plasma was assayed with both methods (r = 0.94), but the mouse leptin assay underestimated rat leptin in plasma. Within- and between-run CVs were 2.4% to 5.7%. Plasma leptin concentrations correlated directly with percentage of body fat, and correlation improved when the results were separated by gender (r = 0.796, P
Databáze: OpenAIRE
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