Application of Zinc Oxide Nanoparticles and Plant Growth Promoting Bacteria Reduces Genetic Impairment under Salt Stress in Tomato (Solanum lycopersicum L. ‘Linda’)
| Autor: | Guller Ozkan, Halil İbrahim Öztürk, Arash Hosseinpour, Péter Poczai, Kamil Haliloglu, Kağan Tolga Cinisli, Alireza Pour-Aboughadareh |
|---|---|
| Přispěvatelé: | Botany, Embryophylo |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Lactobacillus casei POTENTIAL APPLICATIONS ZnO-NP PGPB WHEAT GENOTOXICITY Plant Science Bacillus subtilis Rhizobacteria 01 natural sciences TRITICUM-AESTIVUM Food science lcsh:Agriculture (General) salt stress DNA methylation biology Chemistry Bacillus pumilus fungi 1184 Genetics developmental biology physiology food and beverages 414 Agricultural biotechnology FOLIAR SPRAY 04 agricultural and veterinary sciences DEGRADATION 11831 Plant biology biology.organism_classification genomic instability lcsh:S1-972 RAPD Salinity 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Solanum Agronomy and Crop Science SALINITY STRESS Bacteria RHIZOBACTERIA 010606 plant biology & botany Food Science |
| Zdroj: | Agriculture, Vol 10, Iss 521, p 521 (2020) Agriculture Volume 10 Issue 11 |
| ISSN: | 2077-0472 |
| Popis: | Salinity is an edaphic stress that dramatically restricts worldwide crop production. Nanomaterials and plant growth-promoting bacteria (PGPB) are currently used to alleviate the negative effects of various stresses on plant growth and development. This study investigates the protective effects of different levels of zinc oxide nanoparticles (ZnO-NPs) (0, 20, and 40 mg L&minus 1) and PGPBs (no bacteria, Bacillus subtilis, Lactobacillus casei, Bacillus pumilus) on DNA damage and cytosine methylation changes in the tomato (Solanum lycopersicum L. &lsquo Linda&rsquo ) seedlings under salinity stress (250 mM NaCl). Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RA) and Randomly Amplified Polymorphic DNA (RAPD) approaches were used to analyze changes in cytosine methylation and to determine how genotoxic effects influence genomic stability. Salinity stress increased the polymorphism rate assessed by RAPD, while PGPB and ZnO-NPs reduced the adverse effects of salinity stress. Genomic template stability was increased by the PGPBs and ZnO-NPs application this increase was significant when Lactobacillus casei and 40 mg L&minus 1 of ZnO-NPs were used.A decreased level of DNA methylation was observed in all treatments. Taken together, the use of PGPB and ZnO-NPs had a general positive effect under salinity stress reducing genetic impairment in tomato seedlings. |
| Databáze: | OpenAIRE |
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