Cytotoxicity and Gelatinolytic Activity of a New Silicon-Based Endodontic Sealer
| Autor: | Rodrigo de Menezes Valentim, Aline de Almeida Neves, Edson Jorge Lima Moreira, Ana Paula Moraes, Thais Accorsi-Mendonça, Gustavo De-Deus, Emmanuel João Nogueira Leal Silva |
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| Rok vydání: | 2015 |
| Předmět: |
Silicon
Materials science Cell Survival Blotting Western Gelatin Zymography Biomedical Engineering Biophysics Bioengineering Matrix metalloproteinase Root Canal Filling Materials Biomaterials Mice Western blot medicine Animals Cytotoxic T cell Dimethylpolysiloxanes Viability assay Cytotoxicity medicine.diagnostic_test Epoxy Resins 3T3 Cells General Medicine Molecular biology Blot Drug Combinations Matrix Metalloproteinase 9 Cell culture Matrix Metalloproteinase 2 Gutta-Percha |
| Zdroj: | Journal of Applied Biomaterials & Functional Materials. 13:376-380 |
| ISSN: | 2280-8000 |
| DOI: | 10.5301/jabfm.5000238 |
| Popis: | Purpose To compare the cytotoxicity, gelatinolytic activity, and protein levels (MMP-2 and MMP-9) produced by 3T3 fibroblasts cells after stimulation with GuttaFlow 2 and AH Plus. Methods 3T3 fibroblasts were incubated with elutes of GuttaFlow 2 and AH Plus for 24 h. The cytotoxicity of tested materials was determined using the MTT and the LDH assay. Supernatants of cell cultures incubated with sealers were collected to determine the levels of MMP-2 and MMP-9 gelatinolytic activity by gelatin zymography. Cell lysates were used to determine MMP-2 and MMP-9 protein levels by Western Blot. Data were analyzed using ANOVA and Tukey test (PResults AH Plus showed significantly less cell viability (mitochondrial activity of cells) than GuttaFlow 2 (P0.05). Specific characterization of MMPs demonstrated that GuttaFlow 2 seemed not to affect MMP-2 levels compared with the control group, while AH Plus had elevated gelatinolytic activity and protein levels of MMP-2 as confirmed by quantitative measurements. No detectable gelatinolytic activity or protein levels of MMP-9 (92 kDa) was observed in any tested group. Conclusions GuttaFlow 2 did not showed cytotoxic effects and did not induce MMP-2 or MMP-9 expression. |
| Databáze: | OpenAIRE |
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