Analysis of a novel human gene, LOC92912, over-expressed in hypopharyngeal tumours
| Autor: | Alberto Zambrano, Atefeh Seghatoleslam, Gitali Ganguli, Régine Millon, Joseph Abecassis, Anne Cromer, Bohdan Wasylyk, Manuela Argentini |
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| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cytoplasm
MESH: Cytoskeletal Proteins MESH: Amino Acid Sequence Ubiquitin-conjugating enzyme MESH: Base Sequence Biochemistry MESH: Peptide Mapping 0302 clinical medicine Peptide mass fingerprinting Cloning Molecular Cytoskeleton 0303 health sciences Differential display Microfilament Proteins MESH: Carcinoma Squamous Cell 3. Good health 030220 oncology & carcinogenesis Carcinoma Squamous Cell Protein Binding MESH: Computational Biology MESH: Hypopharyngeal Neoplasms MESH: Cell Line Tumor Molecular Sequence Data Biophysics Biology MESH: Actins Peptide Mapping 03 medical and health sciences MESH: Microfilament Proteins MESH: Gene Expression Profiling Cell Line Tumor medicine Humans MESH: Protein Binding MESH: Cloning Molecular Amino Acid Sequence Molecular Biology Gene Actin MESH: Genes Neoplasm 030304 developmental biology Hypopharyngeal Neoplasms MESH: Humans MESH: Molecular Sequence Data Base Sequence Gene Expression Profiling MESH: Cytoplasm Computational Biology [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology medicine.disease Head and neck squamous-cell carcinoma Molecular biology Actins Cytoskeletal Proteins MESH: Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Ubiquitin-Conjugating Enzymes Genes Neoplasm |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2006, 339 (1), pp.422-9. ⟨10.1016/j.bbrc.2005.11.026⟩ |
| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1016/j.bbrc.2005.11.026⟩ |
| Popis: | International audience; We have identified by differential display a number of novel genes that are expressed in hypopharyngeal head and neck squamous cell carcinoma. We report here the characterisation of one of these novel human genes, LOC92912, that encodes a protein of 375 amino acids. The protein contains a RWD domain, a coiled-coil, and an E2 ubiquitin conjugating enzyme domain. LOC92912 is upregulated in about 85% of tumour samples. It is expressed in tumour masses and in invasive epithelium, and is located in the cytoplasm of cells. To gain insights into its functions, we identified potential interacting partners by immunoaffinity purification of the flag tagged protein followed by MALDI peptide mass fingerprinting mass spectrometry. Actin and six actin-binding proteins were unambiguously identified as potential interacting partners, suggesting that LOC92912's functions may be linked with the cytoskeleton. This novel human gene may represent a new target for cancer therapeutics. |
| Databáze: | OpenAIRE |
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