Bio and soft-imprinting lithography on bacterial cellulose films

Autor: C. Fernández-Sánchez, Soledad Roig-Sanchez, Anna Laromaine, Anna Roig
Přispěvatelé: Ministerio de Ciencia e Innovación (España), Generalitat de Catalunya, European Cooperation in Science and Technology
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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Popis: Bacterial cellulose (BC) is a biocompatible polysaccharide produced by bacteria currently used in packaging, cosmetics, or health care. A highly attractive feature of BC is the possibility of patterning the BC pellicle during its biosynthesis, a concept coined as bio-lithography. BC-patterned films have demonstrated improved properties for cellular-guided growth, implant protection, or wound dressing. However, aspects such as the diversity and size of the features patterned, how those features withstand postprocessing steps, or if large areas can be patterned remain unanswered. Gathering knowledge on these characteristics could extend the use of patterned cellulose-based materials in emerging fields such as transient devices, nanogenerators, or microfluidics. Here, we show that bio-lithographed BC films present good-quality micropatterned features for various motifs (wells, pillars, and channels) in a wide range of sizes (from 200 to 5 μm) and areas as large as 70 cm2. Besides, we have studied the fidelity of the motifs and the fiber organization for wet, supercritical, and oven-dried films. When wells and pillars were patterned, the x and y dimensions were faithfully replicated in the wet and dried samples, but only wet and supercritically dried films afforded mold accuracy in the z-direction. In addition, x/z ratio should be carefully considered for obtaining self-standing pillars. Finally, we compared bio-lithography and soft-imprint lithography. In the latter case, fiber alignment was not observed and the depth of the resulting features dramatically decreased; however, this technique allowed us to produce submicron features that remain after the rewetting of the BC films.
Authors acknowledge financial support from the Spanish Ministry of Science and Innovation through the RTI2018-096273-B-I00 project, the ‘Severo Ochoa’ Programme for Centres of Excellence in R&D (CEX2019-000917-S) and the PhD scholarship of S.R.-S. (BES-2016-077533). The Generalitat de Catalunya projects, 2017SGR765 and 2017SGR1771 are also acknowledged. The authors also express their gratitude to the technical services of ICMAB (SC facilities, SEM, AFM), UAB (microscopy facilities) and ICN2 (electron microscopy). This work used the Spanish ICTS Network MICRONANOFABS, which is partly supported by the Spanish Ministry of Science and Innovation. The authors participate in the CSIC Interdisciplinary Platform for Sustainable Plastics toward a Circular Economy, SUSPLAST and in the Aerogels COST ACTION (CA 18125). The authors very much appreciate the critical revision of A. Mihi (ICMAB) and the stamp provided by his group. This work has been performed within the framework of the doctoral program in materials science of UAB.
Databáze: OpenAIRE