Apicidin biosynthesis is linked to accessory chromosomes in Fusarium poae isolates

Autor:	Anne Johnston, Hai D. T. Nguyen, David P. Overy, Linda J. Harris, Thomas E. Witte, Amanda Sproule, Anne Hermans, Christopher N. Boddy, Jeremy R. Dettman
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Fusarium Canada Biology QH426-470 Peptides Cyclic DNA sequencing Chromosomes chemistry.chemical_compound Fungal plant pathogens Biosynthetic gene clusters Gene cluster Fusarium poae Genetics Metabolomics Apicidin Accessory chromosomes Gene B chromosome Mass spectrometry Research Secondary metabolites Chromosome food and beverages Genomics biology.organism_classification Beauvericin chemistry TP248.13-248.65 Biotechnology
Zdroj:	BMC Genomics, Vol 22, Iss 1, Pp 1-18 (2021) BMC Genomics
ISSN:	1471-2164
Popis:	BackgroundFusarium head blight is a disease of global concern that reduces crop yields and renders grains unfit for consumption due to mycotoxin contamination.Fusarium poaeis frequently associated with cereal crops showing symptoms of Fusarium head blight. While previous studies have shownF. poaeisolates produce a range of known mycotoxins, including type A and B trichothecenes, fusarins and beauvericin, genomic analysis suggests that this species may have lineage-specific accessory chromosomes with secondary metabolite biosynthetic gene clusters awaiting description.MethodsWe examined the biosynthetic potential of 38 F. poaeisolates from Eastern Canada using a combination of long-read and short-read genome sequencing and untargeted, high resolution mass spectrometry metabolome analysis of extracts from isolates cultured in multiple media conditions.ResultsA high-quality assembly of isolate DAOMC 252244 (Fp157) contained four core chromosomes as well as seven additional contigs with traits associated with accessory chromosomes. One of the predicted accessory contigs harbours a functional biosynthetic gene cluster containing homologs of all genes associated with the production of apicidins. Metabolomic and genomic analyses confirm apicidins are produced in 4 of the 38 isolates investigated and genomic PCR screening detected the apicidin synthetase geneAPS1in approximately 7% of Eastern Canadian isolates surveyed.ConclusionsApicidin biosynthesis is linked to isolate-specific putative accessory chromosomes inF. poae. The data produced here are an important resource for furthering our understanding of accessory chromosome evolution and the biosynthetic potential ofF. poae.
Databáze:	OpenAIRE
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