Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters
| Autor: | Konstantin Kanofsky, Fabian Machens, Reinhard Hehl, Jendrik Rusche, Lea Eilert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
DNA
Plant Arabidopsis Electrophoretic Mobility Shift Assay Plant Science Regulatory Sequences Nucleic Acid Pep25 Transcriptional regulation Parsley protoplasts Gene Expression Regulation Plant Genes Reporter Arabidopsis thaliana Electrophoretic mobility shift assay Transient gene expression Binding site Promoter Regions Genetic Transcription factor Gene Reporter gene Binding Sites biology Arabidopsis Proteins fungi Promoter General Medicine biology.organism_classification Cell biology DNA-Binding Proteins Original Article Agronomy and Crop Science Transcription Factors |
| Zdroj: | Plant Cell Reports |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | Key message WRKY50 from A. thaliana requires WT-boxes at target gene promoters for activation and binding. Abstract Based on the genome-wide prediction of WRKY50 target genes and the similarity of a WRKY50 binding site to WT-boxes in microbe-associated molecular pattern (MAMP)-responsive cis-regulatory modules (CRM), four WT-box containing CRMs from the promoter region of three WRKY50 target genes were investigated for their interaction with WRKY50. These target genes are DJ1E, WRKY30 and ATBBE4. Two of the four CRMs, one from DJ1E and one from WRKY30, were able to activate reporter gene expression in the presence of WRKY50. Activation requires the WT-boxes GGACTTTT, GGACTTTG from DJ1E and GGACTTTC from WRKY30. WRKY50 does not activate a second CRM from WRKY30 and the CRM from ATBBE4, both containing the WT-box TGACTTTT. In vitro gel-shift assays demonstrate WT-box-specific binding of the WRKY50 DNA-binding domain to all four CRMs. This work shows a high flexibility of WRKY50 binding site recognition beyond the classic W-box TTGACC/T. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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