Contribution of Swarming Motility to Dissemination in a Pseudomonas aeruginosa Murine Skin Abscess Infection Model
| Autor: | Shannon R. Coleman, Robert E. W. Hancock, Daniel Pletzer |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology Mutant Swarming (honey bee) Swarming motility Motility medicine.disease_cause Microbiology Major Articles and Brief Reports Mice 03 medical and health sciences In vivo medicine Animals Immunology and Allergy Pseudomonas Infections biology Pseudomonas aeruginosa musculoskeletal neural and ocular physiology Biofilm food and beverages Skin Diseases Bacterial biochemical phenomena metabolism and nutrition equipment and supplies biology.organism_classification Abscess 3. Good health 030104 developmental biology Infectious Diseases Biofilms bacteria Peptides Bacteria |
| Zdroj: | J Infect Dis |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiaa778 |
| Popis: | Swarming motility in Pseudomonas aeruginosa is a multicellular adaptation induced by semisolid medium with amino acids as a nitrogen source. By phenotypic screening, we differentiated swarming from other complex adaptive phenotypes, such as biofilm formation, swimming and twitching, by identifying a swarming-specific mutant in ptsP, a metabolic regulator. This swarming-deficient mutant was tested in an acute murine skin abscess infection model. Bacteria were recovered at significantly lower numbers from organs of mice infected with the ∆ptsP mutant. We also tested the synthetic peptide 1018 for activity against different motilities and efficacy in vivo. Treatment with peptide 1018 mimicked the phenotype of the ∆ptsP mutant in vitro, as swarming was inhibited at low concentrations ( |
| Databáze: | OpenAIRE |
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