Physiological and TMT-labeled proteomic analyses reveal important roles of sugar and secondary metabolism in Citrus junos under cold stress
| Autor: | Ni Yang, Ruping Hou, Li Li, Gongwei Qin, Jinglong Jiang, Dekuan Ding, Jiarui Deng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
0301 basic medicine China Citrus Starch Biophysics Secondary Metabolism Biochemistry 03 medical and health sciences chemistry.chemical_compound food Stress Physiological Secondary metabolism Sugar Plant Proteins 030102 biochemistry & molecular biology biology Phenylpropanoid Chemistry Cold-Shock Response food and beverages Fructose biology.organism_classification Citrus junos food.food 030104 developmental biology Seedling Sugars Rootstock |
| Zdroj: | Journal of Proteomics. 237:104145 |
| ISSN: | 1874-3919 |
| DOI: | 10.1016/j.jprot.2021.104145 |
| Popis: | Citrus junos is a widely used citrus grafting rootstock in china because of its excellent tolerance to cold stress. However, the physiological and molecular mechanisms underlying this process remain unknown. In this study, physiological and tandem mass tag-based proteomic analyses were performed to elucidate the mechanism of the Citrus junos response to cold stress. Physiological data showed that severe cold stress decreased photosynthetic parameters and caused cell membrane damage and membrane lipid peroxidation in Citrus junos leaves compared to the control. A total of 6, 678 distinct proteins species were identified, and 413 proteins species were significantly differentially accumulated in the leaves of Citrus junos seedling after cold stress. Bioinformatics analysis revealed that the differentially abundance protein species mainly related to the starch and sucrose metabolism, secondary metabolites biosynthesis and phenylpropanoid biosynthesis in the leaves of Citrus junos seedling after cold stress. Further physiological assays showed that the contents of soluble starch, fructose, glucose and phenols were significantly increased in Citrus junos leaves after cold stress. Collectively, our data reveals that sugar and secondary metabolism could play important roles in Citrus junos in response to cold stress. |
| Databáze: | OpenAIRE |
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