The effect of oestradiol on the acid-soluble nucleotides of rat uterus
| Autor: | Janet M. Oliver, A.E. Kellie |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Purine
History Uracil Nucleotides Uterus Cytosine Nucleotides Education chemistry.chemical_compound Cytosine nucleotide Adenine nucleotide In vivo medicine Animals Nucleotide Perchloric acid chemistry.chemical_classification Chromatography Estradiol Adenine Nucleotides Nucleotides Articles Chromatography Ion Exchange Guanine Nucleotides Stimulation Chemical Rats Computer Science Applications medicine.anatomical_structure Biochemistry chemistry Depression Chemical Female Chromatography Thin Layer Uracil nucleotide |
| Zdroj: | Biochemical Journal. 119:187-191 |
| ISSN: | 0306-3283 |
| DOI: | 10.1042/bj1190187 |
| Popis: | 1. Techniques have been developed to measure the concentrations of the ribonucleotides of the immature rat uterus in vivo. Tissue was frozen rapidly in liquid nitrogen, ground to a fine powder, dispersed in frozen perchloric acid and thawed slowly. Nucleotides were separated from other acid-soluble constituents on short columns of polyethyleneimine-cellulose and the mixture was resolved into individual nucleotides by two-dimensional thin-layer chromatography on polyethyl-eneimine-cellulose plates. 2. The nucleotides of immature rat uterus consisted of approximately 75% of ATP–ADP, 10–12% each of GTP–GDP and UTP–UDP and less than 2% of CTP. 3. Injection of oestradiol (5μg) promoted a linear decrease in the amounts of purine nucleotides to approximately 60% of control values in 4–5h, followed by a return to greater than control values in 8–10h. Concentrations of the pyrimidine nucleotides remained constant for 4–6h and then increased to 200% of control at 12h after hormone treatment. |
| Databáze: | OpenAIRE |
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