Erythropoietin is produced by tubular cells of the rat kidney
| Autor: | Nega Beru, Salim K. Mujais, Theodore N. Pullman, Eugene Goldwasser |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty ATPase Biophysics Stimulation Nephron Biology Biochemistry Rats Sprague-Dawley hemic and lymphatic diseases Internal medicine Gene expression medicine Animals RNA Messenger Erythropoietin Adenosine Triphosphatases chemistry.chemical_classification Kidney Messenger RNA Reverse Transcriptase Polymerase Chain Reaction urogenital system Antimutagenic Agents Cobalt Cell Biology General Medicine Rats Blotting Southern Kidney Tubules Enzyme medicine.anatomical_structure Endocrinology chemistry biology.protein Sodium-Potassium-Exchanging ATPase medicine.drug |
| Zdroj: | Cell Biochemistry and Biophysics. 30:153-166 |
| ISSN: | 1559-0283 1085-9195 |
| DOI: | 10.1007/bf02737888 |
| Popis: | The cellular site of erythropoietin (epo) production within the mammalian kidney is still not completely understood. In the present study, we examined the expression of epo mRNA in microdissected rat nephron segments by RT-PCR after induction of epo expression with cobalt chloride. Erythropoietin mRNA was not detected in nephron segments from saline injected rats. In cobalt chloride injected animals, epo mRNA was found in the majority of samples from the cortical region of the nephron, PCT, and CAL. Medullary tubule preparations (MCT and MAL) were mostly negative for epo mRNA, and glomeruli were uniformly negative. The induction of epo transcripts in tubular cells by cobalt chloride was paralleled by stimulation of the major transport enzyme in the kidney, namely, Na-K ATPase in a tubular profile similar to that of induction of epo transcripts. These results support some earlier findings that epo gene expression in response to cobalt salt stimulation of rat kidney occurs in transporting tubular epithelial cells. |
| Databáze: | OpenAIRE |
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