Simultaneous Determination of Ciprofloxacin and Ofloxacin in Animal Tissues with the Use of Capillary Electrophoresis with Transient Pseudo-Isotachophoresis
| Autor: | Rafał Głowacki, Krystian Purgat, Izabella Kośka, Paweł Kubalczyk |
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| Přispěvatelé: | Department of Environmental Chemistry, Faculty of Chemistry, University of Lodz, Pomorska 163, 90-236 Lodz, Poland, Doctoral School of Exact and Natural Sciences, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland, pawel.kubalczyk@chemia.uni.lodz.pl, krystian.purgat@edu.uni.lodz.pl, rafal.glowacki@chemia.uni.lodz.pl, izabella.koska@edu.uni.lodz.pl |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Calibration curve
Liquid-Liquid Extraction capillary electrophoresis Pharmaceutical Science Organic chemistry Article antibiotics Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis QD241-441 ciprofloxacin Drug Discovery medicine ofloxacin Animals Sample preparation Physical and Theoretical Chemistry Dichloromethane Chromatography Isotachophoresis Chemistry transient pseudo-isotachophoresis Extraction (chemistry) Electrophoresis Capillary Electrophoresis Chemistry (miscellaneous) extraction Molecular Medicine Ofloxacin medicine.drug |
| Zdroj: | Molecules Volume 26 Issue 22 Molecules, Vol 26, Iss 6931, p 6931 (2021) |
| Popis: | We have developed a precise and accurate method for the determination of ciprofloxacin and ofloxacin in meat tissues. Our method utilizes capillary electrophoresis with a transient pseudo-isotachophoresis mechanism and liquid–liquid extraction during sample preparation. For our experiment, a meat tissue sample was homogenized in pH 7.00 phosphate buffer at a ratio of 1:10 (tissue mass: buffer volume g/mL). The extraction of each sample was carried out twice for 15 min with 600 µL of a mixture of dichloromethane and acetonitrile at a 2:1 volume ratio. We then conducted the electrophoretic separation at a voltage of 16 kV and a temperature of 25 °C using a background electrolyte of 0.1 mol/L phosphate–borate (pH 8.40). We used the UV detection at 288 nm. The experimentally determined LOQs for ciprofloxacin and ofloxacin were 0.27 ppm (0.8 nmol/g tissue) and 0.11 ppm (0.3 nmol/g tissue), respectively. The calibration curves exhibited linearity over the tested concentration range of 2 to 10 nmol/g tissue for both analytes. The relative standard deviation of the determination did not exceed 15%, and the recovery was in the range of 85–115%. We used the method to analyze various meat tissues for their ciprofloxacin and ofloxacin contents. |
| Databáze: | OpenAIRE |
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