Intestinal lysozyme releases Nod2 ligand(s) to promote the intestinal mucosal adjuvant activity of cholera toxin
| Autor: | Haifang Wang, Xueying Shen, Xiaojiao Zheng, Qin Zhang, Zhihua Liu, Ying Pan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cholera Toxin T Follicular Helper Cells Plasma Cells Nod2 Signaling Adaptor Protein Ligands medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Adjuvants Immunologic Antigen NOD2 medicine Animals Intestinal Mucosa General Environmental Science Cholera toxin Ligand (biochemistry) Intestinal epithelium digestive system diseases 030104 developmental biology medicine.anatomical_structure chemistry Immunoglobulin G 030220 oncology & carcinogenesis Paneth cell Muramidase General Agricultural and Biological Sciences Acetylmuramyl-Alanyl-Isoglutamine Muramyl dipeptide |
| Zdroj: | Science China Life Sciences. 64:1720-1731 |
| ISSN: | 1869-1889 1674-7305 |
| DOI: | 10.1007/s11427-020-1862-8 |
| Popis: | Commensal bacteria boost serum IgG production in response to oral immunization with antigen and cholera toxin (CT) in a manner that depends on Nod2 (nucleotide-binding oligomerization domain-containing protein 2). In this study, we examined the role of intestinal lysozyme (Lyz1) in adjuvant activity of CT. We found that Lyz1 released Nod2 ligand(s) from bacteria. Lyz1 deficiency reduced the level of circulating Nod2 ligand in mice. Lyz1 deficiency also reduced the production of IgG and T-cellspecific cytokines after oral immunization in mice. Supplementing Lyz1-deficient mice with MDP restored IgG production. Furthermore, overexpression of Lyz1 in intestinal epithelium boosted the antigen-specific IgG response induced by CT. Collectively, our results indicate that Lyz1 plays an important role in mediating the immune regulatory effect of commensal bacteria through the release of Nod2 ligand(s). |
| Databáze: | OpenAIRE |
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