Synthesis of Lewis X epitopes on plant N-glycans
| Autor: | Jan H.G. Cordewener, Dion E.A. Florack, Johannes P. F. G. Helsper, Thamara Hesselink, Dirk Bosch, Gerard J. A. Rouwendal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Glycan
Spectrometry Mass Electrospray Ionization dc-sign Molecular cloning transgenic plants Biochemistry Epitope Analytical Chemistry Epitopes antigen Antigen Polysaccharides Tandem Mass Spectrometry Tobacco expression Animals Humans Beta-galactosidase Fucosylation fuc-tix chemistry.chemical_classification biology alpha-1 3-fucosyl-transferase molecular-cloning Organic Chemistry General Medicine Fucosyltransferases Galactosyltransferases Plants Genetically Modified alpha-1 DC-SIGN carbohydrates (lipids) PRI Bioscience schistosoma-mansoni chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization beta-galactosidases biology.protein carbohydrate epitopes 3-fucosyl-transferase Glycoprotein |
| Zdroj: | Carbohydrate Research : an international journal 344 (2009) 12 Carbohydrate Research : an international journal, 344(12), 1487-1493 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/j.carres.2009.05.003 |
| Popis: | Glycoproteins from tobacco line xFxG1, in which expression of a hybrid beta-(1--4)-galactosyltransferase (GalT) and a hybrid alpha-(1--3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (Le(X)) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid beta-(1--4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant beta-galactosidase activity. |
| Databáze: | OpenAIRE |
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