Escherichia coli induces bovine neutrophil cell death independent from caspase-3/-7/-1, but with phosphatidylserine exposure prior to membrane rupture
| Autor: | Christopher J. Guérin, Peter Vandenabeele, Sofie Notebaert, Filip Boyen, Dieter Demon, Evelyne Meyer, Koen Breyne, Quinten Remijsen, Kristel Demeyere |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
INVOLVEMENT
CLEARANCE BLOOD Neutrophils Interleukin-1beta Apoptosis Neutrophil Activation chemistry.chemical_compound 0302 clinical medicine MILK Bovine neutrophil Caspase 7 0303 health sciences Caspase 3 Caspase 1 SUBCLINICAL MASTITIS Pyroptosis NF-kappa B NETosis Phosphatidylserine Cell biology Caspases Necroptosis Poly(ADP-ribose) Polymerases Cell death EXPRESSION Programmed cell death Phagocytosis Immunology Phosphatidylserines Biology EXTRACELLULAR TRAPS 03 medical and health sciences MAMMARY-GLAND Escherichia coli Animals APOPTOTIC CELLS 030304 developmental biology Innate immune system General Veterinary Interleukin-6 Biology and Life Sciences EXTERNALIZATION chemistry Cattle Reactive Oxygen Species 030215 immunology |
| Zdroj: | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY Veterinary Immunology and Immunopathology; Vol 153 |
| ISSN: | 0165-2427 |
| Popis: | Neutrophils are essential for the innate immune response against bacterial pathogens and play a key role during the early phases of infection, including mastitis and endometritis in cows. When directly challenged with bacteria, neutrophils undergo phagocytosis induced cell death (PICD). The molecular mechanisms of this cell death modality are poorly understood, especially for bovine neutrophils. Therefore, this study aimed to determine the mechanisms and hallmarks of PICD in bovine neutrophils after in vitro challenge with Escherichia coli (E. coli). Our data show that various apoptotic hallmarks such as blebbing, chromatin condensation and executioner caspase (C)-3/-7 activity are only observed during constitutive bovine neutrophil apoptosis. In contrast, bovine neutrophil PICD is characterized by production of reactive oxygen species (ROS), pro-inflammatory C-1 activation, nuclear factor (NF)-κB activation, and interleukin (IL)-1β and IL-6 secretion. Nevertheless, under both conditions these phagocytes undergo cell death with the exposure of phosphatidylserine (PS). Although PS exposure is generally attributed to the anti-inflammatory features of executioner caspase-dependent apoptosis, it surprisingly preceded plasma membrane rupture during bovine neutrophil PICD. Moreover, C-1 inhibition strongly affected IL-1β production but not the PICD kinetics. This indicates that the secretion of the latter pro-inflammatory cytokine is a bystander effect rather than a regulator of PICD in bovine neutrophils, in marked contrast to the IL-1β-dependent pyroptosis reported for macrophages. |
| Databáze: | OpenAIRE |
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