Fiber enhanced Raman spectroscopic analysis as a novel method for diagnosis and monitoring of diseases related to hyperbilirubinemia and hyperbiliverdinemia
| Autor: | Mathias W. Pletz, Di Yan, Robert Domes, Timea Frosch, Torsten Frosch, Jürgen Popp, Christian Domes |
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| Rok vydání: | 2016 |
| Předmět: |
Erythrocytes
Cirrhosis Bilirubin 02 engineering and technology Spectrum Analysis Raman 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound symbols.namesake Electrochemistry medicine Humans Environmental Chemistry Optical Fibers Spectroscopy Hyperbilirubinemia Biliverdin Chemistry Biliverdine 010401 analytical chemistry Infant Newborn Bile Pigments Jaundice 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Red blood cell medicine.anatomical_structure symbols Hemoglobin medicine.symptom 0210 nano-technology Raman spectroscopy |
| Zdroj: | The Analyst. 141:6104-6115 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c6an01670g |
| Popis: | Fiber enhanced resonance Raman spectroscopy (FERS) is introduced for chemically selective and ultrasensitive analysis of the biomolecules hematin, hemoglobin, biliverdin, and bilirubin. The abilities for analyzing whole intact, oxygenated erythrocytes are proven, demonstrating the potential for the diagnosis of red blood cell related diseases, such as different types of anemia and hemolytic disorders. The optical fiber enables an efficient light-guiding within a miniaturized sample volume of only a few micro-liters and provides a tremendously improved analytical sensitivity (LODs of 0.5 μM for bilirubin and 0.13 μM for biliverdin with proposed improvements down to the pico-molar range). FERS is a less invasive method than the standard ones and could be a new analytical method for monitoring neonatal jaundice, allowing a precise control of the unconjugated serum bilirubin levels, and therefore, providing a better prognosis for newborns. The potential for sensing very low concentrations of the bile pigments may also open up new opportunities for cancer research. The abilities of FERS as a diagnostic tool are explored for the elucidation of jaundice with different etiologies including the rare, not yet well understood diseases manifested in green jaundice. This is demonstrated by quantifying clinically relevant concentrations of bilirubin and biliverdin simultaneously in the micro-molar range: for the case of hyperbilirubinemia due to malignancy, infectious hepatitis, cirrhosis or stenosis of the common bile duct (1 μM biliverdin together with 50 μM bilirubin) and for hyperbiliverdinemia (25 μM biliverdin and 75 μM bilirubin). FERS has high potential as an ultrasensitive analytical technique for a wide range of biomolecules and in various life-science applications. |
| Databáze: | OpenAIRE |
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