Factors Involved in the Functional Motor Recovery of Rats with Cortical Ablation after GH and Rehabilitation Treatment: Cortical Cell Proliferation and Nestin and Actin Expression in the Striatum and Thalamus

Autor: José María Criado, A.S. Riolobos, Antonio de la Fuente, Jesús Devesa, Pablo Devesa, Natalia Rodríguez, Margarita Heredia, Virginia Sánchez Robledo
Přispěvatelé: Universidade de Santiago de Compostela. Departamento de Fisioloxía
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
striatum
Gene Expression
Striatum
lcsh:Chemistry
Nestin
0302 clinical medicine
Traumatic brain injury
Thalamus
Cortex (anatomy)
Neural plasticity
lcsh:QH301-705.5
Spectroscopy
traumatic brain injury
Motor Cortex
General Medicine
Computer Science Applications
neurogenesis
medicine.anatomical_structure
medicine.symptom
actin
Motor cortex
neural plasticity
medicine.medical_specialty
Neurogenesis
Catalysis
Article
Inorganic Chemistry
Lesion
03 medical and health sciences
Internal medicine
thalamus
Neuroplasticity
medicine
nestin
Animals
Physical and Theoretical Chemistry
Molecular Biology
Growth hormone
Actin
Cell Proliferation
business.industry
Organic Chemistry
Recovery of Function
medicine.disease
Actins
Corpus Striatum
Rats
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
nervous system
Brain Injuries
growth hormone
business
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
Volume 20
Issue 22
Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname
Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela
Universidad de Santiago de Compostela (USC)
International Journal of Molecular Sciences, Vol 20, Iss 22, p 5770 (2019)
ISSN: 1422-0067
DOI: 10.3390/ijms20225770
Popis: Previously we demonstrated, in rats, that treatment with growth hormone (GH) and rehabilitation, carried out immediately after a motor cortical ablation, significantly improved the motor affectation produced by the lesion and induced the re-expression of nestin in the contralateral motor cortex. Here we analyze cortical proliferation after ablation of the frontal motor cortex and investigate the re-expression of nestin in the contralateral motor cortex and the role of the striatum and thalamus in motor recovery. The rats were subjected to ablation of the frontal motor cortex in the dominant hemisphere or sham-operated and immediately treated with GH or the vehicle (V), for five days. At 1 dpi (days post-injury), all rats received daily injections (for four days) of bromodeoxyuridine and five rats were sacrificed at 5 dpi. The other 15 rats (n = 5/group) underwent rehabilitation and were sacrificed at 25 dpi. GH induced the greatest number of proliferating cells in the perilesional cortex. GH and rehabilitation produced the functional recovery of the motor lesion and increased the expression of nestin in the striatum. In the thalamic ventral nucleus ipsilateral to the lesion, cells positive for nestin and actin were detected, but this was independent on GH. Our data suggest that GH-induced striatal nestin is involved in motor recovery This research was funded by Foundation Foltra (Teo, Spain), grant number 2018-8 SI
Databáze: OpenAIRE
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