Biocatalysed Synthesis of Chiral Amines: Continuous Colorimetric Assays for Mining Amine-Transaminases
| Autor: | Véronique de Berardinis, Egon Heuson, Adrien Debard, Franck Charmantray, Thierry Gefflaut, Léa Gourbeyre, Virgil Hélaine, Jean-Louis Petit |
|---|---|
| Přispěvatelé: | SIGMA Clermont (SIGMA Clermont), Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
010405 organic chemistry Transamination [CHIM.ORGA]Chemical Sciences/Organic chemistry Enantioselective synthesis Substrate (chemistry) Hypotaurine [CHIM.CATA]Chemical Sciences/Catalysis 010402 general chemistry 01 natural sciences Combinatorial chemistry Catalysis 0104 chemical sciences chemistry.chemical_compound chemistry Sulfite Reagent Amine gas treating Titration |
| Zdroj: | Catalysis Science & Technology Catalysis Science & Technology, Royal Society of Chemistry, 2021, 11 (3), pp.904-911. ⟨10.1039/D0CY02070B⟩ Catalysis Science & Technology, 2021, 11 (3), pp.904-911. ⟨10.1039/D0CY02070B⟩ |
| ISSN: | 2044-4753 |
| Popis: | International audience; In the course of our research aimed at the design of new biocatalytic processes for the enantioselective synthesis of chiral amines, we have developed new continuous assays for the screening of amine-transaminases collections. These assays are based on the use of hypotaurine as irreversible amino donor. This β-aminosulfinic acid is converted upon transamination into 2-oxoethylsulfinic acid, which instantaneously decomposes into acetaldehyde and sulfite ions that can be easily detected by spectrophotometry using Ellman's reagent. Two complementary assays were developed based on this titration method. Firstly, a direct assay allowed detecting various transaminases able to use hypotaurine as amino donor. In a second coupled assay, L-alanine is used as generic donor substrate of aminetransaminases and is regenerated using an auxiliary hypotaurine-transaminase. The powerful and complementary nature of both assays was demonstrated through the screening of a collection of 549 amine-transaminases from biodiversity, thus allowing the discovery of a variety of valuable new biocatalysts for use in synthetic processes. |
| Databáze: | OpenAIRE |
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