Effect of prolactin on phospholipid synthesis in organ cultures of fetal rat lung
| Autor: | Yolande F. Smith, Darlene K. Mullon, Paul Hamosh, Margit Hamosh, Laura L. Richardson |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
medicine.medical_specialty
Biophysics Phospholipid Biology Biochemistry Dexamethasone chemistry.chemical_compound Fetus Organ Culture Techniques Endocrinology Pulmonary surfactant Phosphatidylcholine Internal medicine medicine Glycerol Animals Choline Lung Phospholipids Phosphatidylglycerol Rats Inbred Strains Prolactin Rats chemistry medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 751:166-174 |
| ISSN: | 0005-2760 |
| DOI: | 10.1016/0005-2760(83)90171-6 |
| Popis: | We have used lung organ cultures to investigate the effects of prolactin on the synthesis of surfactant phospholipids in the fetal lung. Lung explants prepared from 18-day-old rat fetuses were maintained in culture for 48 h in serum-free medium with two different preparations of prolactin (2 μg/ml —NIH, ovine lot P-S-13 and Sigma, lot 19C-0396). In addition, lung explants were cultured for an initial 24 h with dexamethasone (10−6 M) followed by 24 h of culture with prolactin. Incorporation of surfactant precursors [methyl-3H]choline and [U-14C]glycerol, was measured after incubation for 4 h in the absence of hormones. Exposure of the explants to prolactin, NIH or Sigma, for 48 h led to a significant increase in the levels of total phospholipid −41 and 58% (P < 0.001), respectively. Culture of the lung expiants for the first 24 h with dexamethasone followed by an additional 24 h exposure to prolactin, NIH or Sigma, led to a further increase in the level of total phospholipid, 105 and 112% (P < 0.001) and disaturated PC, 260 and 300% (P < 0.001), respectively. Exposure of lung expiants to prolactin led to a significantly higher incorporation of [methyl-3H]choline into total phospholipid and disaturated PC, 32–35 and 42–45% (P < 0.001), respectively. [U-14C]Glycerol incorporation was significantly higher after culture in prolactin containing medium: 30 and 40% higher into total phospholipid (P < 0.001) and 77 and 105% (P < 0.001) higher into phosphatidylglycerol — the second largest component of lung surfactant. Culture for 24 h with dexamethasone, followed by an additional 24 h with prolactin, led to a further increase in the incorporation of labeled choline and glycerol into surfactant phospholipids: choline incorporation into phospholipid and disaturated PC was 63 and 67% (P < 0.001) and 71 and 75% (P < 0.001), respectively, higher than control; glycerol incorporation was 60 and 72% (P < 0.001) and 192 and 322% (P < 0.001) higher into total phospholipid and phosphatidylglycerol, respectively, than controls. We conclude that: 1, Prolactin acts directly on the fetal rat lung by stimulating the synthesis of surfactant phospholipids. 2, Enhancement of the prolactin effect by dexamethasone is suggested by a further increase in lung phospholipid and disaturated PC levels as well as in the incorporation of surfactant precursors in the presence of both hormones. |
| Databáze: | OpenAIRE |
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