Urease immobilized electrospun PVA/chitosan nanofibers with improved stability and reusability characteristics: an application for removal of urea from artificial blood serum
| Autor: | Nur Kutlu, İlyas Deveci, Yasemin İspirli Doğaç, Mustafa Teke |
|---|---|
| Přispěvatelé: | MÜ, Fen Fakültesi, Kimya Bölümü, MÜ, Muğla Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, Kutlu, Nur, Doğaç, Yasemin İspirli, Teke, Mustafa |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Urease Nanofibers 01 natural sciences Biochemistry Polyvinyl alcohol Nanocomposites Chitosan chemistry.chemical_compound Immobilization Blood serum Blood Substitutes 010608 biotechnology Urea Nanocomposite biology Electrospinning 010405 organic chemistry Hydrolysis General Medicine Nanofiber Enzymes Immobilized 0104 chemical sciences Kinetics chemistry Polyvinyl Alcohol biology.protein Biotechnology Nuclear chemistry |
| Popis: | deveci, ilyas/0000-0002-2753-2301; ispirli dogac, yasemin/0000-0001-8616-0280 WOS: 000523151600001 PubMed ID: 32233958 Electrospun polyvinyl alcohol (PVA)/Chitosan nanofibers were successfully prepared and were used as carriers for the first time in urease immobilization. Also, urease immobilized electrospun PVA/Chitosan nanofibers were applied for the removal of urea from artificial blood serum by recycled reactor. The nanofibers were optimized and synthesized by electrospinning technique according to the operational parameters. The morphology and structure of the nanofibers were characterized by scanning electron microscopy (SEM), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and thermogravimetric analysis (TGA). Urease was immobilized on the nanofibers by adsorption and crosslinking methods. According to immobilization results, nanofiber enhanced urease stability properties like thermal stability, pH stability, and reusability. Urease immobilized electrospun PVA/Chitosan nanofiber protected its activity by 85% after 10 uses and 45% after 20 uses. Urea removal rates of artificial blood serum were as follows: 100% at 1st cycle, 95% at 2nd, 3rd and 4th cycles; 85% at the 5th cycle; 76% at the 6th cycle, and 65% at the last three cycles. Mugla Sitki Kocman University Scientific Research ProjectMugla Sitki Kocman University [:13/182, 16/038] This work was supported by a grant from the Mugla Sitki Kocman University Scientific Research Project (Project No:13/182 and 16/038). |
| Databáze: | OpenAIRE |
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