Trolox‐induced cardiac differentiation is mediated by the inhibition of Wnt/β‐catenin signaling in human embryonic stem cells
| Autor: | Min Young Lee, Hojin Lee, Joong Sun Kim, Woochul Chang, Mu Seog Choe, Seung Pil Yun, Kyoung Min Baek, Chang Min Bae, Han Cheol Yeo, Kyung Seob Lim, Ho Jae Han, In-Sik Shin |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cardiac differentiation Vitamin E medicine.medical_treatment Regulator Wnt signaling pathway Cell Biology General Medicine Inhibitory postsynaptic potential Embryonic stem cell Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry 030220 oncology & carcinogenesis embryonic structures medicine Trolox Induced pluripotent stem cell |
| Zdroj: | Cell Biology International. 43:1505-1515 |
| ISSN: | 1095-8355 1065-6995 |
| DOI: | 10.1002/cbin.11200 |
| Popis: | Cardiac differentiation of human pluripotent stem cells may be induced under chemically defined conditions, wherein the regulation of Wnt/β-catenin pathway is often desirable. Here, we examined the effect of trolox, a vitamin E analog, on the cardiac differentiation of human embryonic stem cells (hESCs). 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) significantly enhanced cardiac differentiation in a time- and dose-dependent manner after the mesodermal differentiation of hESCs. Trolox promoted hESC cardiac differentiation through its inhibitory activity against the Wnt/β-catenin pathway. This study demonstrates an efficient cardiac differentiation method and reveals a novel Wnt/β-catenin regulator. |
| Databáze: | OpenAIRE |
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