Purification of a growth factor related to platelet-derived growth factor and a type β transforming growth factor secreted by mouse neuroblastoma cells. A general strategy for the purification of basic polypeptide growth factors
| Autor: | T. M. J. Van Oostwaard, S.W. de Laat, E.J.J. van Zoelen, I. Koornneef, Alie Feyen, W. Kruijer, A.J.M. van den Eijnden-van Raaij |
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| Rok vydání: | 1989 |
| Předmět: |
Blood Platelets
Platelet-derived growth factor medicine.medical_treatment Size-exclusion chromatography Biology Biochemistry Mice Neuroblastoma chemistry.chemical_compound Tumor Cells Cultured medicine Animals Bioassay Platelet Growth Substances Beta (finance) Molecular Biology Chromatography High Pressure Liquid Platelet-Derived Growth Factor Regulation of gene expression Chromatography Growth factor Cell Biology Gene Expression Regulation chemistry Transforming Growth Factors Chromatography Gel Peptides Research Article Transforming growth factor |
| Zdroj: | Biochemical Journal. 257:375-382 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2570375 |
| Popis: | A general strategy was developed for the purification of basic polypeptide growth factors. This method is a combination of gel filtration, weak-cation-exchange h.p.l.c. and reverse-phase h.p.l.c., separating the proteins according to size, charge and hydrophobicity respectively. All steps are carried out at low pH with exclusively volatile acidic buffer solutions. The sterile conditions and easy removal of salt by freeze-drying facilitate the detection of the growth factors by biological assays. By using this method, homogeneous preparations of two basic growth factors were purified in high yield from mouse-neuroblastoma-Neuro-2A-cell-conditioned medium. It is shown that these purified factors are biochemically and immunologically related to platelet-derived growth factor and type beta transforming growth factor from human platelets. |
| Databáze: | OpenAIRE |
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