Enantioselective oxidative stress and oxidative damage caused by Rac- and S-metolachlor to Scenedesmus obliquus
| Autor: | Xiaoqiang Zhang, Huijun Liu, Shaoting Du, Yina Zhang, Weidan Cai, Yilu Xia |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chlorophyll
Environmental Engineering Stereochemistry Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology Vacuole 010501 environmental sciences medicine.disease_cause 01 natural sciences Superoxide dismutase chemistry.chemical_compound Acetamides medicine Environmental Chemistry 0105 earth and related environmental sciences chemistry.chemical_classification 021110 strategic defence & security studies Reactive oxygen species biology Herbicides Superoxide Dismutase Public Health Environmental and Occupational Health Stereoisomerism General Medicine General Chemistry APX Catalase Pollution Chloroplast Oxidative Stress chemistry biology.protein Biophysics Reactive Oxygen Species Oxidative stress Water Pollutants Chemical Scenedesmus |
| Zdroj: | Chemosphere. 173 |
| ISSN: | 1879-1298 |
| Popis: | The rational use and environmental security of chiral pesticides has gained the interest of many researchers. The enantioselective effects of Rac- and S-metolachlor on oxidative stress in Scenedesmus obliquus were determined in this study. Stronger green fluorescence was observed in response to S-metolachlor treatment than to Rac-metolachlor treatment, suggesting that more reactive oxygen species (ROS) were stimulated by S-metolachlor. ROS levels following S-metolachlor treatment were 1.92-, 8.31-, and 1.08-times higher than those observed following Rac-metolachlor treatment at 0.1, 0.2, and 0.3 mg/L, respectively. Superoxide dismutase (SOD) and catalase (CAT) were stimulated with increasing herbicide concentrations, with S-metolachlor exhibiting a greater effect. Oxidative damage in terms of chlorophyll (Chl) content, cellular membrane permeability, and cellular ultrastructures of S. obliquus were investigated. Chla and Chlb contents in algae treated with Rac-metolachlor were 2–6-fold higher than those in algae treated with S-metolachlor at 0.1, 0.2, and 0.3 mg/L. The cellular membrane permeability of algae exposed to 0.3 mg/L Rac- and S-metolachlor was 6.19- and 42.5-times that of the control. Correlation analysis implied that ROS are the major factor responsible for the oxidative damage caused by Rac- and S-metolachlor. Damage to the chloroplasts and cell membrane of S. obliquus, low production of starch granules, and an increased number of vacuoles were observed upon ultrastructural morphology analysis by transmission electron microscope. These results indicate that S-metolachlor has a greater effect on S. obliquus than Rac-metolachlor. |
| Databáze: | OpenAIRE |
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