Protective Effect ofPinus koraiensisNeedle Water Extract Against Oxidative Stress in HepG2 Cells and Obese Mice
| Autor: | Sae Bom Won, Juhae Kim, Ga-young Jung, Young Hye Kwon, Young Shin Chung, Eun Kyung Hong |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment Glutathione reductase Mice Obese Medicine (miscellaneous) medicine.disease_cause Antioxidants Lipid peroxidation Mice chemistry.chemical_compound In vivo Internal medicine medicine Animals Humans Obesity Cytotoxicity Mice Inbred ICR Nutrition and Dietetics biology Plant Extracts Hep G2 Cells Catalase Pinus In vitro Plant Leaves Oxidative Stress Glutathione Reductase Endocrinology Liver Biochemistry chemistry biology.protein Lipid Peroxidation Full Communications Oxidative stress |
| Zdroj: | Journal of Medicinal Food. 16:569-576 |
| ISSN: | 1557-7600 1096-620X |
| DOI: | 10.1089/jmf.2012.2665 |
| Popis: | Needles of pine species are rich in polyphenols, which may exert beneficial effects on human health. The present study was conducted to evaluate the in vitro and in vivo antioxidant effects of Pinus koraiensis needle water extracts (PKW). HepG2 cells were pretreated with various concentrations of PKW (from 10(-3) to 1 mg/mL) and oxidative stress was induced by tert-butyl hydroperoxide (t-BOOH). In the animal model, male ICR mice were fed a high-fat diet for 6 weeks to induce obesity, and then mice were continually fed a high-fat diet with or without orally administered PKW (400 mg/kg body weight) for 5 weeks. Pretreatment with PKW prevented significant increases in cytotoxicity and catalase activity induced by t-BOOH in HepG2 cells. Similarly, the catalase protein expression levels elevated by t-BOOH were abrogated in cells pretreated with PKW. In mice fed a high-fat diet, PKW significantly increased hepatic activities of catalase and glutathione reductase and lower lipid peroxidation levels were observed in the liver and kidney of mice with PKW supplementation. The present study demonstrates that PKW protects against oxidative stress in HepG2 cells treated with t-BOOH and in mice fed a high-fat diet. |
| Databáze: | OpenAIRE |
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