Hepatitis B genotypes/subgenotypes and MHR variants among Moroccan chronic carriers

Autor: Khadija Rebbani, Soumaya Benjelloun, Omar Derdabi, Hanane Salih Alj, Isabelle Chemin, M. Benazzouz, Younes Cherradi, Abdellah Essaid El Feydi, Christian Trepo, Bouchra Kitab, Ikram Brahim, Fabien Zoulim, Rajaa Afifi, Sayeh Ezzikouri
Přispěvatelé: Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP), Service d'hépatologie et de gastroentérologie, Hospices Civils de Lyon (HCL)-Hôtel-Dieu, Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), IFR Laennec (IL), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Hépatites Virales [Casablanca, Maroc] (LHV), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre de Recherche en Cancérologie de Lyon (CRCL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2011
Předmět:
Male
HBsAg
medicine.disease_cause
MESH: Genotype
0302 clinical medicine
MESH: Aged
80 and over
Gene Frequency
Genotype
MESH: Phylogeny
Phylogeny
MESH: Aged
Aged
80 and over
0303 health sciences
MESH: Middle Aged
virus diseases
Hepatitis B
Middle Aged
3. Good health
MESH: Hepatitis B virus
Morocco
Infectious Diseases
HBeAg
MESH: Young Adult
Carrier State
030211 gastroenterology & hepatology
Female
MESH: Carrier State
Viral load
Sequence Analysis
Microbiology (medical)
MESH: DNA Primers
Adult
Hepatitis B virus
MESH: Mutation
Adolescent
MESH: Hepatitis B
Chronic
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
03 medical and health sciences
MESH: Sequence Analysis
Young Adult
Hepatitis B
Chronic
Antigen
medicine
MESH: Gene Frequency
Humans
Gene
030304 developmental biology
Aged
DNA Primers
MESH: Adolescent
MESH: Humans
Hepatitis B Surface Antigens
MESH: Adult
medicine.disease
Virology
digestive system diseases
MESH: Male
MESH: Hepatitis B Surface Antigens
MESH: Morocco
Mutation
MESH: Female
Zdroj: Journal of Infection
Journal of Infection, WB Saunders, 2011, 63 (1), pp.66-75. ⟨10.1016/j.jinf.2011.05.007⟩
ISSN: 1532-2742
0163-4453
DOI: 10.1016/j.jinf.2011.05.007⟩
Popis: International audience; OBJECTIVES: The aim of this study was to determine the prevalence of Hepatitis B Virus (HBV) genotypes, subgenotypes, HBV surface antigen (HBsAg) subtypes and naturally occurring mutations in Major Hydrophilic region (MHR) of HBsAg among Moroccan patients with chronic HBV infection. METHODS: The study included 200 patients chronically infected with HBV. The HBV genotypes, subgenotypes, HBsAg subtypes and MHR variants were determined by direct sequencing of the HBV surface (S) gene and phylogenetic analysis. RESULTS: The S gene was successfully amplified in 134 patients. The mean age was 40.6 ± 12.2 years. Genotype D was predominant (90%, 120/134) and genotype A was less frequent (10%, 14/134). Genotype D strains belonged to subgenotypes D7 (70.8%, 85/120), D1 (25.8%, 31/120) and D2 (0.9%, 1/120). Three strains (2.5%) could not be classified in any subgenotype of genotype D. All genotype A strains belonged to subgenotype A2. HBsAg subtypes found were ayw2 (82.1%, 110/134), adw2 (10.4%, 14/134), ayw3 (3%, 4/134) and ayw4 (3%, 4/134). The global prevalence of MHR variants was 15% (20/134) with substitution P120T/S the most frequent (3.7%, 5/134). The occurrence of MHR variants was significantly associated with advancing age (>40 years) (p = 0.003) and independent of sex, HBeAg status, viral load, genotype, subgenotype and HBsAg subtype. CONCLUSIONS: This study provides the first description of predominance of HBV subgenotype D7/subtype ayw2 among Moroccan HBV chronic carriers. It also showed a significant prevalence of naturally occurring MHR variants in Morocco.
Databáze: OpenAIRE
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