Active stress kinases in proliferating endothelial cells associated with cytoskeletal structures
| Autor: | Marianne Hamel, Linda J. Lowe-Krentz, Mark D. Cipolle, Daniela Kanyi |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Physiology
p38 mitogen-activated protein kinases Cell Culture Techniques Fluorescent Antibody Technique Biology Pulmonary Artery Microfilament p38 Mitogen-Activated Protein Kinases Cell Line Focal adhesion chemistry.chemical_compound Animals Cytochalasin Cytoskeleton Cell Proliferation Mitogen-Activated Protein Kinase Kinases Focal Adhesions Microscopy Confocal Kinase JNK Mitogen-Activated Protein Kinases Colocalization Endothelial Cells Cell Biology General Medicine Vinculin Molecular biology Cell biology Capillaries Nocodazole chemistry Adrenal Medulla biology.protein Cattle Protein Kinases |
| Zdroj: | Endothelium : journal of endothelial cell research. 13(3) |
| ISSN: | 1062-3329 |
| Popis: | It has become increasingly clear that stress-activated protein kinases have cytoplasmic substrates in addition to well-established transcription factor substrates in cell nuclei. The present study documented specific cytoplasmic locations of these enzymes in proliferating vascular cells. Immunofluorescent staining for active c-jun NH2-terminal kinase (JNK), the precipitation of JNK with microfilaments, and the loss of fiber-associated active JNK after cytochalasin treatment, but not nocodazole treatment, together indicate that active JNK is associated with stress fibers. The lack of complete scaffold colocalization and the total lack of immediate upsteam kinase colocalization along with the inability of JNK inhibitors to alter JNK-microfilament associations suggest that the microfilament association is not simply involved in enzyme activation. In addition, active p38 was found along with vinculin in focal adhesions. Although the p38 in focal adhesions could also be disrupted by cytochalasin treatment, it remained stable after nocodazole treatment. These results support the hypothesis that vascular cell stress kinase enzymes are important for signal transduction in the cytoplasm. The localization of active stress-activated protein kinases to specific cytoskeletal structures in proliferating cells suggests that subsets of these enzymes are involved in signal transduction to and/or from the cytoskeleton under conditions that include vascular cell proliferation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|