Elevated hepatic glucocorticoid receptor expression during liver regeneration in rats
| Autor: | O. Dobozy, András Fülöp, Árpád Kiss, Bálint Madarász, Karabélyos C, András Falus, Csaba Szalai, Ákos Hadházi, Kornélia Varjú, Katalin Klenjánszki |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty medicine.medical_treatment Biology Pathology and Forensic Medicine Glucocorticoid receptor Receptors Glucocorticoid Internal medicine Gene expression medicine Animals Rats Wistar Receptor General Medicine Immunohistochemistry Liver regeneration Liver Regeneration Rats Steroid hormone Endocrinology Oncology Liver Female Hepatectomy Glucocorticoid medicine.drug |
| Zdroj: | Pathology oncology research : POR. 5(2) |
| ISSN: | 1219-4956 |
| Popis: | In rats within the first week of partial hepatectomy reconstruction of the normal histological structure of the liver already starts. To approach the possible role of endogenous glucocorticoids in the process of regeneration we measured the changes in the expression of steroid glucocorticoid receptor gene after various regeneration intervals. After partial hepatectomy, between 0.5–168 hours from the surgery, the gene expression (mRNA) of glucocorticoid receptor was determined by reverse transcription followed by PCR and normalized to that of glycerolphoshate dehydrogenase. Two peaks of glucocorticoid receptor mRNA were detected first, bet ween 3 and 6 hours (first peak) and a second between 24 and 36 hours. Immunoreactive glucocorticoid receptor was detected by immunohistochemistry using monoclonal anti-glucocorticoid receptor. Three days after the surgery immunohistochemical studies showed substantially more immunoreactive GcR protein in the regenerated liver than in the controls. These semiquantitative data provide evidence suggesting elevation of glucocorticoid receptor expression during regeneration of liver at mRNA and protein levels. |
| Databáze: | OpenAIRE |
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