Interactive regulation of signalling pathways in bone cells: possible modulation of PGE2-stimulated adenylyl cyclase activity by protein kinase C
| Autor: | Pauline R.M. Dobson, T.J. Martin, Roslin Russell, Jane M. Moseley, Barry L. Brown, A. D. Short, V.P. Michelangeli |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Parathyroid hormone Biology Cyclase ADCY10 Dinoprostone Adenylyl cyclase chemistry.chemical_compound Salmon Internal medicine medicine Tumor Cells Cultured Animals Molecular Biology Protein kinase C Protein Kinase C Osteosarcoma ADCY9 Cell Biology Rats Endocrinology chemistry Phorbol Tetradecanoylphorbol Acetate Cattle Cyclase activity Adenylyl Cyclases Signal Transduction Vasoactive Intestinal Peptide |
| Zdroj: | Biochimica et biophysica acta. 1052(2) |
| ISSN: | 0006-3002 |
| Popis: | We have reported previously that tumour-promoting phorbol esters modulate both basal and vasoactive intestinal polypeptide (VIP)-stimulated adenylyl cyclase activity in GH 3 (an established pituitary cell line). Here, we probe the receptor and cell specificity of this response. Experiments were performed in the presence of isobutylmethylxanthine. Unlike the response in GH 3 cells, the tumour-promoting phorbol ester (tetradecanoyl phorbol acetate (TPA)) did not affect either basal adenylyl cyclase activity nor VIP-stimulated activity in the rat osteosarcoma subclones UMR 106-01 and UMR 106-06. In addition, the cyclase responses to parathyroid hormone (PTH), and, in the case of UMR 106-06, to calcitonin were unaffected by tumour-promoting phorbol ester. However, prostaglandin E 2 -stimulated cyclase activity in both of these subclones was attenuated in a dose-dependent manner. |
| Databáze: | OpenAIRE |
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