AMPA/kainate receptors modulate the survival in vitro of embryonic brainstem cells
| Autor: | Norbert König, M. Bardoul, M.-J. Drian |
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| Rok vydání: | 1997 |
| Předmět: |
Agonist
Programmed cell death medicine.drug_class Cell Survival Kainate receptor AMPA receptor Biology Pharmacology Rats Sprague-Dawley chemistry.chemical_compound Embryonic and Fetal Development Developmental Neuroscience Receptors Kainic Acid medicine DNQX Excitatory Amino Acid Agonists Animals Receptors AMPA Receptor Cell growth S100 Proteins Antagonist Embryo Mammalian Rats chemistry Bromodeoxyuridine Phosphopyruvate Hydratase Excitatory Amino Acid Antagonists Developmental Biology Brain Stem |
| Zdroj: | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 15(6) |
| ISSN: | 0736-5748 |
| Popis: | This study aimed at analyzing the involvement of (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate (AMPA/kainate) receptors in the survival of cultured rat embryonic brainstem cells, dissociated on embryonic day 14. The cell number was estimated after pharmacological manipulation of the receptors by exposure to agonists or antagonists. The developmental stage at the moment of drug application was critical for cell survival. We observed after 8 days in vitro a much stronger decrease in the number of gamma-enolase-positive cells when the cultures were treated for 3 days with the antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX) starting on the day of plating than when DNQX was added after 5 days in vitro. Conversely, exposure to the agonists (RS)-2-amino-3-(3-hydroxy-5-tri-fluoromethyl-4-isoxazolyl)-propion ic acid (T-AMPA) or kainate for 3 days significantly reduced cell survival only when the treatment was initiated after 5 days in vitro. Survival of S-100-positive cells was not affected after exposure to either agonists or antagonists. Neither agonist nor antagonist treatment modified cell proliferation, as assessed by 5-bromo-2'-deoxyuridine (BrdU) staining, suggesting that the decrease in the number of gamma-enolase-positive cells is essentially due to cell death. If some of the processes we observed in vitro correspond to analogous events in vivo, then exposure to excitatory amino acid receptor agonists or antagonists at critical stages of embryogenesis may alter the development of the central nervous system. |
| Databáze: | OpenAIRE |
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