Androgens augment pulmonary responses to ozone in mice
| Autor: | Ross S. Osgood, Stephanie A. Shore, David I. Kasahara, Youngji Cho, Hiroki Tashiro |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Physiology IL‐1α Reproductive Hormones 030204 cardiovascular system & hematology lcsh:Physiology Flutamide chemistry.chemical_compound 0302 clinical medicine Interleukin-1alpha Lung Methacholine Chloride Original Research Sex Characteristics lcsh:QP1-981 medicine.diagnostic_test respiratory system Neutrophil Infiltration Androgens Cytokines medicine.symptom Bronchoalveolar Lavage Fluid Toxins Pollutants and Chemical Agents medicine.drug medicine.medical_specialty Immunology Inflammation 03 medical and health sciences Ozone Airway hyperresponsiveness Physiology (medical) Internal medicine medicine Respiratory Hypersensitivity Animals Asthma Respiratory Conditions Disorder and Diseases business.industry Interleukin-6 Androgen Antagonists Pneumonia castration medicine.disease respiratory tract diseases Androgen receptor Mice Inbred C57BL Oxidative Stress Bronchoalveolar lavage Endocrinology Castration chemistry Respiratory Mechanics Methacholine business Corticosterone Orchiectomy 030217 neurology & neurosurgery Hormone |
| Zdroj: | Physiological Reports Physiological Reports, Vol 7, Iss 18, Pp n/a-n/a (2019) |
| ISSN: | 2051-817X |
| Popis: | Ozone causes airway hyperresponsiveness, a defining feature of asthma, and is an asthma trigger. In mice, ozone‐induced airway hyperresponsiveness is greater in males than in females, suggesting a role for sex hormones in the response to ozone. To examine the role of androgens in these sex differences, we castrated 4‐week‐old mice. Controls underwent sham surgery. At 8 weeks of age, mice were exposed to ozone (2ppm, 3 h) or room air. Twenty‐four hours later, mice were anesthetized and measurements of airway responsiveness to inhaled aerosolized methacholine were made. Mice were then euthanized and bronchoalveolar lavage was performed. Castration attenuated ozone‐induced airway hyperresponsiveness and reduced bronchoalveolar lavage cells. In intact males, flutamide, an androgen receptor inhibitor, had similar effects to castration. Bronchoalveolar lavage concentrations of several cytokines were reduced by either castration or flutamide treatment, but only IL‐1α was reduced by both castration and flutamide. Furthermore, an anti‐IL‐1α antibody reduced bronchoalveolar lavage neutrophils in intact males, although it did not alter ozone‐induced airway hyperresponsiveness. Our data indicate that androgens augment pulmonary responses to ozone and that IL‐1α may contribute to the effects of androgens on ozone‐induced cellular inflammation but not airway hyperresponsiveness. |
| Databáze: | OpenAIRE |
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