Characterization of T-Dependent and T-Independent B Cell Responses to a Virus-like Particle
| Autor: | Can Liu, Lin Lin, Zhaolin Hua, Wen-Hua Liao, Rui Chen, Baidong Hou |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Immunogen T cell T-Lymphocytes Immunology B-cell receptor Naive B cell Biology Antibodies Viral 03 medical and health sciences Mice Virus-like particle medicine Immunology and Allergy Animals Vaccines Virus-Like Particle B cell B-Lymphocytes Staining and Labeling Virology Molecular biology B-1 cell 030104 developmental biology medicine.anatomical_structure Immunoglobulin M Immunoglobulin G biology.protein Proto-Oncogene Proteins c-bcl-6 Antibody Immunologic Memory |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 198(10) |
| ISSN: | 1550-6606 |
| Popis: | Natural pathogens, such as viruses, often induce T-dependent and T-independent Ab responses. However, the activation and differentiation of Ag-specific B cells under these conditions had not been examined in detail. In this study, we used bacterial phage Qβ-derived virus-like particles (Qβ-VLPs) as an immunogen to examine the T-independent and T-dependent phases of the response in mice. Using Qβ-specific cell labeling and enrichment methods developed in this study, we were able to characterize the rare Ag-specific B cells in detail. Surprisingly, we found that Qβ-VLPs could induce Bcl-6 expression in pregerminal center B cells independently of T cell help. In addition, Qβ-VLP–induced T-independent responses could lead to isotype-switched and somatically mutated memory B cells. Finally, in contrast to what has been reported with several other Ags, long-lived IgG+ memory cells were induced by Qβ-VLPs, with IgM+ memory B cells being produced but only evident for a limited time, suggesting that different types of immunogens may preferentially generate or maintain IgM+ versus IgG+ memory B cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|