Injectable, scalable 3D tissue-engineered model of marrow hematopoiesis
| Autor: | Fabien Bonini, Olaia Naveiras, Josefine Tratwal, Sylke Hoehnel, Marco Alessandrini, Patrick Burch, Vasco Campos, Martina Genta, Daniel Naveed Tavakol, Amélie Beduer, Thomas Braschler |
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| Rok vydání: | 2020 |
| Předmět: |
Stromal cell
Biophysics Bone Marrow Cells Bioengineering Stroma 02 engineering and technology ddc:616.07 Biology Models Biological Extramedullary Hematopoiesis Scaffold Biomaterials Mice 03 medical and health sciences Bone Marrow In vivo medicine Animals Minimally invasive Stem Cell Niche Progenitor cell Cell Proliferation 030304 developmental biology 0303 health sciences Regeneration (biology) Mesenchymal stem cell Bone marrow niche Extramedullary hematopoiesis Hematopoietic stem cells Microcarrier Cell Differentiation 021001 nanoscience & nanotechnology Coculture Techniques Hematopoiesis Cell biology Transplantation medicine.anatomical_structure Mechanics of Materials Printing Three-Dimensional Ceramics and Composites Bone marrow 0210 nano-technology |
| Zdroj: | Biomaterials, vol. 232, pp. 119665 Biomaterials, Vol. 232 (2020) P. 119665 |
| ISSN: | 0142-9612 |
| Popis: | Modeling the interaction between the supportive stroma and the hematopoietic stem and progenitor cells (HSPC) is of high interest in the regeneration of the bone marrow niche in blood disorders. In this work, we present an injectable co-culture system to study this interaction in a coherent in vitro culture and in vivo transplantation model. We assemble a 3D hematopoietic niche in vitro by co-culture of supportive OP9 mesenchymal cells and HSPCs in porous, chemically defined collagen-coated carboxymethylcellulose microscaffolds (CCMs). Flow cytometry and hematopoietic colony forming assays demonstrate the stromal supportive capacity for in vitro hematopoiesis in the absence of exogenous cytokines. After in vitro culture, we recover a paste-like living injectable niche biomaterial from CCM co-cultures by controlled, partial dehydration. Cell viability and the association between stroma and HSPCs are maintained in this process. After subcutaneous injection of this living artificial niche in vivo, we find maintenance of stromal and hematopoietic populations over 12 weeks in immunodeficient mice. Indeed, vascularization is enhanced in the presence of HSPCs. Our approach provides a minimalistic, scalable, biomimetic in vitro model of hematopoiesis in a microcarrier format that preserves the HSPC progenitor function, while being injectable in-vivo without disrupting the cell-cell interactions established in vitro. |
| Databáze: | OpenAIRE |
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