PCR method for detection of largemouth bass virus
| Autor: | Ilhan Altinok, Andrew D. Noyes, John M. Grizzle |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
food.ingredient
Iridovirus Molecular Sequence Data Micropterus Aquatic Science Polymerase Chain Reaction Sensitivity and Specificity Virus law.invention Bass (fish) Fish Diseases food Capsid law Animals Ecology Evolution Behavior and Systematics Centrarchidae Polymerase chain reaction biology Base Sequence Gene Amplification biology.organism_classification Virology DNA Virus Infections DNA Viral Bass Capsid Proteins sense organs Pcr method |
| Zdroj: | Diseases of aquatic organisms. 54(1) |
| ISSN: | 0177-5103 |
| Popis: | A polymerase chain reaction (PCR) method was developed for largemouth bass virus (LMBV). This iridovirus can cause a lethal disease of largemouth bass Micropterus salmoides, but also subclinically infects largemouth bass and other species of fishes. Oligonucleotide primers were designed to specifically amplify the major capsid protein gene of LMBV. The protocol for sample processing and PCR provided a method that was more sensitive than cell culture for detection of LMBV in fish. The specific amplification of LMBV also provided an improved method for confirming the identity of cell-culture isolates presumptively identified as LMBV. |
| Databáze: | OpenAIRE |
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