Plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in Morocco
| Autor: | Khalid Zerouali, Hamid Amarouch, Naima El Mdaghri, Jean David Perrier Gros Claude, Mohammed Timinouni, Mohammed Bouchakour, Patrice Courvalin |
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| Přispěvatelé: | Institut Pasteur du Maroc, Réseau International des Instituts Pasteur (RIIP), CHU Ibn Rochd [Casablanca], Faculté des Sciences Aïn Chock [Casablanca] (FSAC), Université Hassan II [Casablanca] (UH2MC), Agents antibactériens, Institut Pasteur [Paris], Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Klebsiella
Nalidixic acid medicine.medical_treatment MESH: beta-Lactamases Quinolones Polymerase Chain Reaction Hospitals University 0302 clinical medicine Prevalence 030212 general & internal medicine MESH: Enterobacteriaceae Infections 0303 health sciences MESH: Microbial Sensitivity Tests biology Enterobacteriaceae Infections General Medicine Enterobacter Enterobacteriaceae 3. Good health Anti-Bacterial Agents Ciprofloxacin Morocco Infectious Diseases medicine.drug Plasmids Microbial Sensitivity Tests Microbiology beta-Lactamases 03 medical and health sciences MESH: Enterobacteriaceae MESH: Plasmids Virology MESH: Anti-Bacterial Agents Drug Resistance Bacterial MESH: Drug Resistance Bacterial medicine Humans MESH: Prevalence MESH: Hospitals University MESH: Humans MESH: Quinolones 030306 microbiology MESH: Polymerase Chain Reaction biology.organism_classification Proteus mirabilis [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Morocco Beta-lactamase Parasitology Enterobacter cloacae |
| Zdroj: | Journal of Infection in Developing Countries Journal of Infection in Developing Countries, Independent, 2010, 4 (12), pp.779-803. ⟨10.3855/jidc.796⟩ Journal of Infection in Developing Countries, 2010, 4 (12), pp.779-803. ⟨10.3855/jidc.796⟩ |
| ISSN: | 2036-6590 1972-2680 |
| DOI: | 10.3855/jidc.796⟩ |
| Popis: | Introduction : Although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or AmpC-type beta-lactamases. Since no data are available in African countries, the prevalence of qnr genes at the University Hospital Ibn Rochd, Casablanca, Morocco, was investigated. Methodology: Between October 2006 and March 2007, the following 39 randomly selected non-duplicate Enterobacteriaceae producing an extended-spectrum beta-lactamase (ESBL), representing 20% of ESBL strains with respect to species and ward origin, were collected: Escherichia coli (n = 16); Klebsiella spp (n = 14); Enterobacter cloacae (n = 8); Proteus mirabilis (n = 1). Antibiotic susceptibility testing was performed according to CLSI guidelines. ESBL detection was performed by the double disc diffusion test. A multiplex PCR was conducted to detect qnrA , qnrB and qnrS genes that were confirmed by sequencing of the PCR product. Results: The estimated overall prevalence of qnr reached 36% (n = 14; qnrA , 10.25%; qnrB , 23.07%; qnrS , 2.56%). Genes were identified in E. coli , Klebsiella and Enterobacter with a respective prevalence of 18.7%, 50% and 62.5%. The qnr genes were detected in nine wards and qnrA1 , qnrB1-B2-B4 and qnrS1 variants were identified. Three genes were identified among nalidixic acid susceptible strains (n = 6); three of those were also susceptible to ciprofloxacin. Among nalidixic acid and ciprofloxacin resistant strains, all strains had qnrB . Conclusions: This study highlights the high prevalence of qnr genes among ESBL strains in the Ibn Rochd CHU, Casablanca. Moreover, qnr were present in quinolone-susceptible strains which could lead to in vivo selection of ciprofloxacin-resistant strains. |
| Databáze: | OpenAIRE |
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