Correlation between Biophysical Properties of Niosomes Elaborated with Chloroquine and Different Tensioactives and Their Transfection Efficiency

Autor: Santiago Grijalvo, Nuseibah Al Qtaish, Ramon Eritja, Gustavo Puras, Margarita Menéndez, Ilia Villate-Beitia, Idoia Gallego, Laura Lagartera, Myriam Sainz-Ramos, José Luis Pedraz
Přispěvatelé: Ministerio de Ciencia e Innovación (España), Eritja Casadellà, Ramón, Eritja Casadellà, Ramón [0000-0001-5383-9334]
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Pharmaceutics, Vol 13, Iss 1787, p 1787 (2021)
Pharmaceutics
Addi. Archivo Digital para la Docencia y la Investigación
Volume 13
Issue 11
Universidad de Cantabria (UC)
Popis: Lipid nanocarriers, such as niosomes, are considered attractive candidates for non-viral gene delivery due to their suitable biocompatibility and high versatility. In this work, we studied the influence of incorporating chloroquine in niosomes biophysical performance, as well as the effect of non-ionic surfactant composition and protocol of incorporation in their biophysical performance. An exhaustive comparative evaluation of three niosome formulations differing in these parameters was performed, which included the analysis of their thermal stability, rheological behavior, mean particle size, dispersity, zeta potential, morphology, membrane packing capacity, affinity to bind DNA, ability to release and protect the genetic material, buffering capacity and ability to escape from artificially synthesized lysosomes. Finally, in vitro biological studies were, also, performed in order to determine the compatibility of the formulations with biological systems, their transfection efficiency and transgene expression. Results revealed that the incorporation of chloroquine in niosome formulations improved their biophysical properties and the transfection efficiency, while the substitution of one of the non-ionic surfactants and the phase of addition resulted in less biophysical variations. Of note, the present work provides several biophysical parameters and characterization strategies that could be used as gold standard for gene therapy nanosystems evaluation.
Authors wish to thank: ICTS “NANBIOSIS”, specifically the Drug Formulation Unit (U10) of the CIBER in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) for the intellectual and technical assistance; SGIker (UPV/EHU) for technical and human support; the Department of Education, University and Research of the Basque Country Government (Consolidated Groups, IT907-16); the Spanish Ministry of Science and Innovation (Grants PID2019-106199RB-C21 and RTI2018-099985-B-I00); also the support of CIBER of Respiratory Diseases (CIBERES-ISCIII) and the Cystic Fibrosis Association of the Basque Country. M.S.-R. thanks the University of the Basque Country (UPV/EHU) for the granted pre-doctoral fellowship (PIF17/79). I.V.-B. thanks the University of the Basque Country (UPV/EHU) for the granted postdoctoral fellowship (call for the Specialization of Doctor Researcher Personnel of the UPV/EHU, grant reference: ESPDOC19/47).
Databáze: OpenAIRE
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